Video: Dialyze small-volume samples with Slide-A-Lyzer MINI Dialysis Devices

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Have you ever wanted to dialyze small-volume samples, but couldn’t risk it with dialysis tubing because of the potential for losing much of your precious sample?

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Protein sample preparation kit for mass spectrometry

Protein Sample Prep Tools for Mass Spectrometry

The new Pierce Mass Spec Sample Prep Kit for Cultured Cells provides the components needed to generate consistent and reproducible protein digests that are directly compatible with LC-MS workflows. The digests do not require further processing, such as C18 clean up or detergent removal.

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Eliminate endotoxins from various protein sources, sizes and charges

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Traditional endotoxin removal methods require significant optimization for every target molecule being purified. Furthermore, ionic methods for binding endotoxins require pH optimization to avoid binding highly charged proteins, and size-exclusion methods to filter endotoxins are not effective for large proteins. In this Product Article about removing endotoxins from samples, our scientists demonstrate how they used

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Removing endotoxins from protein samples

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Endotoxin removal during the development of biological therapeutics is critical for preventing inflammation, tissue death and endotoxin shock. In this Product Article about removing endotoxins, our scientists discuss how they evaluated a spin-column method that is ideal for protein samples.

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Introducing Detergent Removal Resin for Low Concentration Protein Sample Cleanup

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ROCKFORD, Ill. (January 23, 2012) – Thermo Fisher Scientific, the world leader in serving science, today introduced the Thermo Scientific™ HiPPR Detergent Removal Resin for the efficient cleanup of protein and peptide solutions. The resin helps avoid interference in downstream analysis such as ELISAs, isoelectric focusing and mass spectrometry.

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Two New Additions to Microdialysis Product Line

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ROCKFORD, Ill. (January 19, 2012) – Thermo Fisher Scientific, the world leader in serving science, today announced the addition of the Thermo Scientific™ Pierce™ 96-well Microdialysis Plate with a 3.5K molecular weight cut-off. The microdialysis plates are ideal for removing low molecular-weight contaminants, performing small-volume buffer exchange and desalting samples.

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Fast and Effective Subcellular Protein Fractionation Kit

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ROCKFORD, Ill. (January 9, 2012) – Thermo Fisher Scientific, the world leader in serving science, today introduced the Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissue.

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Announcing New High-capacity Endotoxin Removal Resin

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ROCKFORD, Ill. (Jaunuary 5, 2012) – Thermo Fisher Scientific, the world leader in serving science, today introduced the Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin for the cleanup of protein that is injected or transfected into cells or animals.

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Sample prep methods for mass spectrometry

Protein Mass Spectrometry and HPLC

We have added a page on Sample Preparation for Mass Spectrometry to the Protein Methods Library, which details the various aspects of sample preparation for both MALDI- and ESI-based MS analysis.

Slide-A-Lyzer MINI Dialysis Devices for small samples

Slide-A-Lyzer MINI Dialysis Devices

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 3.5K, 10K and 20K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules in volumes from 10µL to 2mL.