No Joke! Express glycoproteins in a human IVT system

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In a new product applications article, Krishna Vattem provides a protocol and demonstrates how the 1-Step Human Coupled in vitro Translation System can be used to express glycoproteins complete with N-linked glycosylation.

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Improved human high-yield in vitro translation kits

Protein Expression Systems

We recently made improvements to our 1-Step Human High-Yield In Vitro Translation Kits, which enable the expression of functional proteins with 10 to 100 times greater yield per milliliter than other mammalian IVT systems.

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Highly active proteins expressed by IVT

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A new product article demonstrates the ability of the Thermo Scientific 1-Step Human High-Yield In Vitro Translation Kit to rapidly express highly functional caspase-3 enzyme, as well as how the system can be used to screen modulators of protein activity.

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In vitro protein expression in a 96-well format

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A new product article demonstrates how to use 96-well microdialysis plates to perform high-yield in vitro translation (IVT), also called cell-free protein expression, with Thermo Scientific Human High-Yield Mini IVT Kits.

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Cell-free protein expression strategies to optimize purification

Our most recent product article describes strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientificâ„¢ 1-Step High Yield IVT protein expression system.

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Got mRNA? Express it by in vitro translation

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A new product article about cell-free protein expression describes a protocol for in vitro protein synthesis from mRNA templates using components of the Thermo Scientific 1-Step Human Coupled IVT Kit (primarily for DNA templates).

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Biomarker breakthroughs with human expressed protein

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Biomarkers are antibodies, receptors, peptides, nucleic acids or any other molecule that indicates a biological condition and are increasingly used in the clinic to:

diagnose a disease (diagnostic biomarkers) determine the overall survival of patients with a specific disease (prognostic biomarkers) identify patients who will positively or negatively respond to a particular treatment (predictive biomarkers).

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Luciferase assay system revolutionizes the luciferase assay

Protein Expression Systems

It’s a fact that luciferase assays have greater sensitivity over other assays that use traditional reporters. However, the Thermo Scientific Pierce Luciferase Assay System delivers flexibility beyond that of standard luciferase assays.

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Express full-length heavy peptides for mass spectrometry analysis

Protein Expression Systems

Thermo Scientific 1-Step Heavy Protein In Vitro Protein Expression Kits enable rapid cell-free expression of recombinant proteins containing stable isotope-labeled (i.e., heavy) amino acids.

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Express proteins in one step

Protein Expression Systems

Our human in vitro translation system is the ideal choice when traditional protein expression methods are unsuccessful and for difficult-to-express proteins.

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