Phosphoprotein profiling by mass spectrometry


This product article, republished from the 2009 volume of Previews magazine, describes the work of researchers who collaborated with our scientists to profile changes in phosphoprotein expression in cancer stem cells using several Thermo Scientific Instruments and Protein Biology Products.

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Generate stable isotope-labeled protein standards for quantitative mass spectrometry


Stable isotope-labeled peptides are routinely used as internal standards for mass spectrometry (MS) quantitation of enzymatically digested protein samples. However, stable isotope-labeled proteins are considered better standards because they control for MS sample-preparation loss and digestion variations. In this Product Article about generating MS standards, our scientists describe using in vitro translation (IVT) as an

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Express full-length heavy peptides for mass spectrometry analysis

Protein Expression Systems

Thermo Scientific 1-Step Heavy Protein In Vitro Protein Expression Kits enable rapid cell-free expression of recombinant proteins containing stable isotope-labeled (i.e., heavy) amino acids.

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Sample prep methods for mass spectrometry

Protein Mass Spectrometry and HPLC

We have added a page on Sample Preparation for Mass Spectrometry to the Protein Methods Library, which details the various aspects of sample preparation for both MALDI- and ESI-based MS analysis.

Strategies for Quantitative Proteomics

Protein Mass Spectrometry and HPLC

The Protein Methods Library now includes a comprehensive overview of Quantitative Proteomics for both discovery and targeted proteomic analyses to understand global proteomic dynamics in a cell, tissue or organism.

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RBS 50 Detergent Concentrate and RBS A375 Acid Neutralizer

Laboratory Cleaning Detergents

Thermo Scientific RBS 50 Detergent is a low-foaming, liquid detergent formulated for use in automatic washers and ultrasonic baths to clean laboratory glassware, stainless steel equipment and hard surfaces such as plastic and porcelain. For additional cleaning power, RBS 50 Detergent can be combined with Thermo Scientific RBS A375 Acid Neutralizer.

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Pierce Detergent Removal Spin Plates

Detergent Removal Resins and Kits

Thermo Scientific Pierce Detergent Removal Resin is now available pre-packed in to 96-well filter spin plates for efficient removal of detergents from multiple samples with minimal sample loss.

Mass Spectrometer Calibration Solutions

Proteomics Reagents for Mass Spectrometry

Thermo Scientific positive and negative ionization calibration standards optimized for Thermoscientific Mass Spectrometers and a heavy peptide mixture for all LC and LC-MS users.

PEPotec SRM Peptide Libraries

Peptide Libraries

Thermo Scientific PEPotec SRM Peptide Libraries provide fully synthetic, crude peptides customized for the development of mid- to high-throughput single reaction monitoring (SRM) and multiple reaction monitoring (MRM) assays in quantitative mass spectrometry (MS) workflows.

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Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit

Peptide Sample Prep Tools for Mass Spectrometry

Thermo Scientific Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit enables fast, selective enrichment of phosphorylated peptides.