Phosphoprotein profiling by mass spectrometry

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This product article, republished from the 2009 volume of Previews magazine, describes the work of researchers who collaborated with our scientists to profile changes in phosphoprotein expression in cancer stem cells using several Thermo Scientific Instruments and Protein Biology Products.

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Generate stable isotope-labeled protein standards for quantitative mass spectrometry

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Stable isotope-labeled peptides are routinely used as internal standards for mass spectrometry (MS) quantitation of enzymatically digested protein samples. However, stable isotope-labeled proteins are considered better standards because they control for MS sample-preparation loss and digestion variations. In this Product Article about generating MS standards, our scientists describe using in vitro translation (IVT) as an

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Express full-length heavy peptides for mass spectrometry analysis

Protein Expression Systems

Thermo Scientific 1-Step Heavy Protein In Vitro Protein Expression Kits enable rapid cell-free expression of recombinant proteins containing stable isotope-labeled (i.e., heavy) amino acids.

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Sample prep methods for mass spectrometry

Protein Mass Spectrometry and HPLC

We have added a page on Sample Preparation for Mass Spectrometry to the Protein Methods Library, which details the various aspects of sample preparation for both MALDI- and ESI-based MS analysis.

Strategies for Quantitative Proteomics

Protein Mass Spectrometry and HPLC

The Protein Methods Library now includes a comprehensive overview of Quantitative Proteomics for both discovery and targeted proteomic analyses to understand global proteomic dynamics in a cell, tissue or organism.

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RBS 50 Detergent Concentrate and RBS A375 Acid Neutralizer

Laboratory Cleaning Detergents

Thermo Scientific RBS 50 Detergent is a low-foaming, liquid detergent formulated for use in automatic washers and ultrasonic baths to clean laboratory glassware, stainless steel equipment and hard surfaces such as plastic and porcelain. For additional cleaning power, RBS 50 Detergent can be combined with Thermo Scientific RBS A375 Acid Neutralizer.

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Pierce Detergent Removal Spin Plates

Detergent Removal Resins and Kits

Thermo Scientific Pierce Detergent Removal Resin is now available pre-packed in to 96-well filter spin plates for efficient removal of detergents from multiple samples with minimal sample loss.

Peptide Calibration Reagent to Optimize Liquid Chromatography Performance

Proteomics Reagents for Mass Spectrometry

ROCKFORD, Ill. (May 10, 2011) – Thermo Fisher Scientific, the world leader in serving science, today announced the availability of the Thermo Scientific Pierce Peptide Retention Time Calibration Mixture for the prediction of peptide retention times on reversed-phase high-performance liquid chromatography (HPLC) columns. The convenient, ready-to-use Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic,

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New Calibration Reagents for Mass Spectrometers

Proteomics Reagents for Mass Spectrometry

ROCKFORD, Ill. (May 3, 2011) – Thermo Fisher Scientific, the world leader in serving science, today introduced four Thermo Scientific Pierce Calibration Solutions for the calibration of Thermo Scientific Mass Spectrometers, including the Thermo Scientific LTQ Orbitrap series and the Thermo Scientific TSQ Vantage series. The Pierce* Calibration Solutions are ready-to-use liquid formulations that consist

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Mass Spectrometer Calibration Solutions

Proteomics Reagents for Mass Spectrometry

Thermo Scientific positive and negative ionization calibration standards optimized for Thermoscientific Mass Spectrometers and a heavy peptide mixture for all LC and LC-MS users.