Have you tried the new Thermo Scientific Pierce Trypsin Protease? This mass spectrometry grade preparation is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
|
|||||||
|
The new Pierce Mass Spec Sample Prep Kit for Cultured Cells provides the components needed to generate consistent and reproducible protein digests that are directly compatible with LC-MS workflows. The digests do not require further processing, such as C18 clean up or detergent removal. 
Use only the high quality solvents to carry proteomic samples through liquid chromatography systems for mass spectrometry analysis. We recently expanded our line of Thermo Scientific LC-MS Solvents to include convenient 1% solutions and new bottle sizes to suit any scale of analysis. Choose the variety and bottle size of formic acid, trifluoroacetic acid, dimethylsulfoxide 
Peptide purification improves the signal-to-noise ratio in MALDI and ESI mass spectrometry analysis during protein sequencing. Thermo Scientific Pierce C18 resins provide excellent recovery for a range for sample volumes and up to 80µg of peptide from protein digests. 
Previously available only by custom order, our new cysteine-reactive Thermo Scientific iodoTMT Reagents and Labeling Kit are now offered as standard product items. 
This product article, republished from the 2009 volume of Previews magazine, describes the work of researchers who collaborated with our scientists to profile changes in phosphoprotein expression in cancer stem cells using several Thermo Scientific Instruments and Protein Biology Products.
Stable isotope-labeled peptides are routinely used as internal standards for mass spectrometry (MS) quantitation of enzymatically digested protein samples. However, stable isotope-labeled proteins are considered better standards because they control for MS sample-preparation loss and digestion variations. In this Product Article about generating MS standards, our scientists describe using in vitro translation (IVT) as an 
Thermo Scientific 1-Step Heavy Protein In Vitro Protein Expression Kits enable rapid cell-free expression of recombinant proteins containing stable isotope-labeled (i.e., heavy) amino acids. 
We have added a page on Sample Preparation for Mass Spectrometry to the Protein Methods Library, which details the various aspects of sample preparation for both MALDI- and ESI-based MS analysis.
The Protein Methods Library now includes a comprehensive overview of Quantitative Proteomics for both discovery and targeted proteomic analyses to understand global proteomic dynamics in a cell, tissue or organism. |
|
||||||
|
|||||||
