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Cell and protein biologists have come to trust our high-grade Surfact-Amps Detergent Solutions for preparing customized cell lysis reagents, wash buffers, and protein solubilization solutions. The 10% (w/v) aqueous solutions are both very pure and convenient to use. 
Remove detergents more completely and rapidly from cell lysates and other processed biological samples using our HiPPR Detergent Removal Spin Columns. The ready-to-use microcentrifuge columns (or resin kit or 96-well filter plates) are designed for use with low-concentration peptide or protein samples. 
The study of protein localization and translocation is important for understanding protein functions and diseases, especially cancers. Subcellular protein fractionation enables protein localization discoveries, enhances detection of low-abundance species, reduces sample complexity and enables the monitoring of physiologic fluxes and redistribution under basal and stimulated/diseased conditions. Tissue samples, however, present an extra level of complexity
Synaptosomes are commonly used to study synaptic function because they contain functional ion channels, receptors, enzymes and proteins, as well as the intact molecular machinery for the release, uptake and storage of neurotransmitters. Signal transmission is highly regulated by neuronal phosphoproteins, and therefore, maintaining protein phosphorylation during synaptosome preparation is essential. In this Product Article 
We are proud to present a new article on protein glycosylation and methods to detect and analyze glycoproteins and glycopeptides, respectively. This article is the most recent addition to the series on post-translational modifications in the Protein Methods Library.
Proteases and phosphatases serve many metabolic and regulatory functions; however, upon cell lysis, the once compartmentally contained enzymes are capable of mass protein degradation and dephosphorylation. 
Our universal nuclease is ideal for cell lysis and a wide variety of other applications. Get the same performance as Benzonase* Nuclease but at a better price. 
Thermo Scientific Pierce Protease and Phosphatase Inhibitor Tablets are now available in multiple combinations, including the only combination protease and phosphatase inhibitor tablet, for protecting your protein from degradation and dephosphorylation.
Neurons have unique morphology and lipid content, making protein extraction from this cell type very challenging. In this Product Article about neuronal protein extraction, our scientists show how the Thermo Scientific N-PER Neuronal Protein Extraction Reagent enables total protein isolation from neuronal tissue or primary cultured cells without adversely affecting protein function. 
ROCKFORD, Ill. (January 23, 2012) – Thermo Fisher Scientific, the world leader in serving science, today introduced the Thermo Scientific™ HiPPR Detergent Removal Resin for the efficient cleanup of protein and peptide solutions. The resin helps avoid interference in downstream analysis such as ELISAs, isoelectric focusing and mass spectrometry. |
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