A new product article about luciferase assay strategies describes how our researchers developed and demonstrated a method to simultaneously assay the expression of two different genes relative to a third (normalizing) gene.
|
|||||||
|
A new product article, summarizes results on the use of three spectrally-resolvable luciferase reporters and high-content immunofluorescence cellular imaging to monitor phenotypic changes in neuronal precursor cell lines following retinoic acid-induced differentiation.
In this new product article, our researchers demonstrate how they performed experiments to confirm the efficacy of Thermo Scientific Pierce Luciferase Dual Assay Kits to spectrally resolve and quantify the activity of two luciferases using a commercial plate reader capable of simultaneous measurement at two different wavelengths. 
If a picture is worth a thousand words, how valuable are a couple videos? Dual-spectral luciferase assays are a great method to monitor the activity of two promoters simultaneously or to study a single promoter over a period of time.
Dual-spectral luciferase reporter assays are a sensitive experimental approach to quantifying gene reporter activity, but distinguishing the individual luciferase signals is tedious work when calculating the luminescence by hand. 
It’s a fact that luciferase assays have greater sensitivity over other assays that use traditional reporters. However, the Thermo Scientific Pierce Luciferase Assay System delivers flexibility beyond that of standard luciferase assays.
Luciferase assays are a powerful way to study gene regulation because of their sensitivity and broad dynamic range. While there are many instruments to use for these and other luciferase-based assays, determining which instrument will work best can be difficult because of their wide feature variability.
Although plasmid-based luciferase assays are powerful tools for studying signal transduction pathways, cells with low transfection efficiency can make signal detection difficult. In this Product Article about performing luciferase assays in Jurkat cells, our scientists demonstrate the effectiveness of using the Thermo Scientific Pierce Luciferase Reporter Systems to transfect Jurkat cells and the Thermo Scientific
The American Museum of Natural History* in New York City recently released a free iPad* app as a companion to their Creatures of Light: Nature’s Bioluminescence exhibition.
Details on using bioluminescence and luciferase reporters in life science research can now be found in a new article in the Protein Methods Library. Luciferase enzymes are increasing used for in vivo imaging, viability assays and reporter gene assays because of their sensitivity and diversity in substrate requirements and signal duration. |
|
||||||
|
|||||||
