Glowing results in one step

Luciferase Reporter Assays

The new Pierce Firefly Luc One-Step Glow Assay Kit expands our growing line of luciferase assay reagents to provide a way to measure firefly luciferase activity in mammalian cell culture using a single reagent-addition step.

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Quantitative co-IP without elution?

Co-Immunoprecipitation and Pull-Down Kits

The new Pierce qIP Protein Interaction Kits use anti- HA or anti-Myc beads (agarose or magnetic) and a sensitive luciferase assay system to co-immunoprecipitate (co-IP) and quantify interactions between epitope-tagged and Tluc-tagged protein pairs expressed in mammalian cells.

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Microplate luminometers for flash-type luciferase assays

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A new product article describes features of selected Thermo Scientific microplate luminometers that make them ideal for high-throughput reporter-gene studies using Thermo Scientific Pierce Luciferase Assays.

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Dual assays? Try triple luciferase assays!

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A new product article about luciferase assay strategies describes how our researchers developed and demonstrated a method to simultaneously assay the expression of two different genes relative to a third (normalizing) gene.

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Triple luciferase assays, cell imaging and neuron development

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A new product article, summarizes results on the use of three spectrally-resolvable luciferase reporters and high-content immunofluorescence cellular imaging to monitor phenotypic changes in neuronal precursor cell lines following retinoic acid-induced differentiation.

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New article on dual-spectral luciferase assays

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In this new product article, our researchers demonstrate how they performed experiments to confirm the efficacy of Thermo Scientific Pierce Luciferase Dual Assay Kits to spectrally resolve and quantify the activity of two luciferases using a commercial plate reader capable of simultaneous measurement at two different wavelengths.

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New dual-spectral luciferase animation videos!

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If a picture is worth a thousand words, how valuable are a couple videos? Dual-spectral luciferase assays are a great method to monitor the activity of two promoters simultaneously or to study a single promoter over a period of time.

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Downloadable dual-spectral luciferase calculator is now available

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Dual-spectral luciferase reporter assays are a sensitive experimental approach to quantifying gene reporter activity, but distinguishing the individual luciferase signals is tedious work when calculating the luminescence by hand.

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Luciferase assay system revolutionizes the luciferase assay

Protein Expression Systems

It’s a fact that luciferase assays have greater sensitivity over other assays that use traditional reporters. However, the Thermo Scientific Pierce Luciferase Assay System delivers flexibility beyond that of standard luciferase assays.

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Luciferase detection instrument guide

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Luciferase assays are a powerful way to study gene regulation because of their sensitivity and broad dynamic range. While there are many instruments to use for these and other luciferase-based assays, determining which instrument will work best can be difficult because of their wide feature variability.

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