Protein glycosylation methods article

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We are proud to present a new article on protein glycosylation and methods to detect and analyze glycoproteins and glycopeptides, respectively. This article is the most recent addition to the series on post-translational modifications in the Protein Methods Library.

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Clean up your protein

Antibody Production and Purification

Our high-capacity endotoxin removal resins reduce endotoxin levels in protein samples by ≥ 99%. The new spin-column format makes it easy to clean up and recover your sample in 1 hour.

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Need help choosing a column size for antibody purification?

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Given the many and various uses of antibodies in laboratory research endeavors, it’s no surprise that there are many available sizes and varieties of affinity purification columns, chromatography cartridges, filter plates and other devices for purifying antibodies easily and quickly at small or large scales.

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GlycoLink Immobilization Kits and Resin

Carbohydrate Immobilization Resins and Kits

The Thermo Scientific GlycoLink IP Kit provides components for effective immunoprecipitation (IP and co-IP) based on small-scale, covalent, affinity-resin immobilization of antibodies and other glycoproteins via oxidized sugar groups.

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Anti-HA Agarose and HA Tag IP/Co-IP Kit

Tag-Based IP and Pull-Down Kits

Thermo Scientific Pierce Anti-HA Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant HA-tagged proteins or with immunoprecipitation and Co-IP assays.

Pierce Protein A/G Magnetic Beads

Pierce Magnetic Beads

Thermo Scientific Pierce Protein A/G Magnetic Beads and IP/Co-IP Kits are high-performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators.

ActivX Fluorophosphonate Probes

Enzyme Active-Site Probes and Enrichment Kits

Thermo Scientific ActivX* Fluorophosphonate (FP) Probes allow covalent labeling of the serine hydrolase active site with affinity, fluorescent or reactive tags to facilitate selective enrichment, detection or multiplex labeling.

Pierce GTPase Enrichment Kits

Enzyme Active-Site Probes and Enrichment Kits

Thermo Scientific Pierce GTPase Enrichment Kits with ActivX* GTP Probes enable selective labeling and enrichment of GTPases including G-protein coupled receptor GTPase subunits. These reagents feature a desthiobiotin (biotin analog) that facilitates capture, clean-up and easy recovery of labeled GTPases using the Pierce Kinase Enrichment Kits.

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Pierce Kinase Enrichment Kits

Enzyme Active-Site Probes and Enrichment Kits

Thermo Scientific Pierce Kinase Enrichment Kits with ActivX* ATP or ADP Probes enable selective labeling and enrichment of ATPases including kinases, chaperones and metabolic enzymes. These reagents feature a desthiobiotin (biotin analog) that facilitates capture, clean-up and easy recovery of labeled kinases using the Pierce Kinase Enrichment Kits.

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Pierce Glutathione Agarose and Kits

GST-tagged Protein Purification Resins and Kits

Thermo Scientific Pierce Glutathione Agarose is a high-capacity, high-performance resin for affinity purification of GST-tagged fusion proteins from cellular lysates.

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