New article about Pierce quantitative immunoprecipitation assays

Quantitative IP in a high-throughput format

By Douglas Hayworth

In a new product article, Dr. Jae Choi and colleagues describe key features of the Pierce qIP Protein Interaction Assay System and then provide example experimental data and demonstrate its use in a high-throughput (HTS) microplate format.

The new Pierce qIP Assays Kits use anti- HA or anti-Myc beads (agarose or magnetic) or coated microplates and a sensitive luciferase assay system to co-immunoprecipitate (co-IP) and quantify interactions between epitope-tagged and Tluc-tagged protein pairs expressed in mammalian cells. The quantitative immunoprecipitation (qIP) system depends on TurboLuc luciferase enzyme (Tluc) to accurately and precisely reflect the abundance of a specific co-IP product without time-consuming gel electrophoresis, Western blot and band densitometry steps.


Read the Article:

Pierce qIP Assay: a novel, HTS-compatible protein-protein interaction assay system

Pierce qIP Assay: a novel, HTS-compatible protein-protein interaction assay system