Pierce qIP Protein Interaction Kits

Quantitative co-IP of expressed protein interaction pairs without elution?

By Douglas Hayworth

The new Pierce qIP Protein Interaction Kits use anti- HA or anti-Myc beads (agarose or magnetic) and a sensitive luciferase assay system to co-immunoprecipitate (co-IP) and quantify interactions between epitope-tagged and Tluc-tagged protein pairs expressed in mammalian cells.

The quantitative immunoprecipitation (qIP) system depends on TurboLuc luciferase enzyme (Tluc) to accurately and precisely reflect the abundance of a specific co-IP product without time-consuming gel electrophoresis, Western blot and band densitometry steps.

Highlights:

  • Quantitative – integrated luciferase assay allows direct measurement of co-IP products
  • Sensitive – bright TurboLuc luciferase allows detection of even weak interactions
  • Time-saving – elution steps and post-IP Western blotting are not required
  • Robust – compatible with mammalian cell lines including 293T, HEK293, NIH3T3, CHO-K1
  • Versatile – the assay platform is highly customizable

Featured Products:

Quantitative Protein Interaction Reagents and Kits

Quantitative Protein Interaction Reagents and Kits

Overview of Pierce qIP Protein Interaction Kits

Overview of Pierce qIP Protein Interaction Kits