10-plex Tandem Mass Tag Reagents for mass spectrometry

Mass tagging to analyze 10 proteomic conditions simultaneously

By Douglas Hayworth

Amine-reactive Thermo Scientific TMT10plex Isobaric Mass Tag Labeling Kits and Reagents enable multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS).

This new 10-plex set (TMT10plex™) of Tandem Mass Tag™ (TMT™) reagents contains ten different isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm and a mass reporter. The reagent set enables up to ten different peptide samples prepared from cells or tissues to be labeled in parallel and then combined for analysis. For each sample, a unique reporter mass (i.e., TMT10 126-131Da) in the low-mass region of the high-resolution MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation and tandem mass spectrometry.

Highlights:

  • Powerful – concurrent MS analysis of multiple samples increases sample throughput
  • Consistent – identical reagent structure and performance among all TMT reagents
  • Robust – increased multiplex capability results in fewer missing quantitative values
  • Efficient – amine-reactive, NHS-ester reagents ensure efficient labeling of all peptides
  • Compatible – optimized for use with high resolution Thermo Scientific MS/MS platforms

View Products:

Amine-reactive, 10-plex Tandem Mass Tag Reagents

Amine-reactive, 10-plex Tandem Mass Tag Reagents

All Thermo Scientific Tandem Mass Tag Reagents

All Thermo Scientific Tandem Mass Tag Reagents