New article on procedures for primary cardiomyocyte isolation

Isolate and culture primary cardiomyocytes easily and speedily

By Douglas Hayworth

In a new product article, Dr. Hai-Yan Wu and colleagues compare available protocols for primary cardiomyocyte isolation from mouse and rat hearts, showcasing the newly developed Pierce Cardiomyocyte Isolation Kit.

Wu et al. compared the efficacy of four methods with respect to enzyme digestion, cell isolation efficiency, and cardiomyocyte function. The new method, commercialized as the Thermo Scientific Pierce Cardiomyocyte Isolation Kit, is a significant improvement over existing isolation protocols, enabling consistently high cell yields exhibiting high viability and improved function as assessed by the spontaneous beating activity of the cardiomyocyte cultures.


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One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts