Improved MEF primary cell isolation
By Douglas Hayworth
In a new product article, Dr. Hai-Yan Wu and colleagues compare available protocols for mouse embryonic fibroblast (MEF) isolation, showcasing the newly developed Pierce MEF Isolation Kit.
Traditional do-it-yourself (DIY) methods for MEF isolation use trypsin. The new method uses an alternative, papain-based procedure for gently dissociating fibroblasts from embryonic mouse tissue (E11-13). The simple and reliable procedure significantly improves the yield and overall cell health and is applicable to a variety of mouse strains, including pathogen-free mice and genetically modified disease mice.
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