New article on in vitro protein expression systems

Highly active proteins expressed by IVT

By Douglas Hayworth

A new product article demonstrates the ability of the Thermo Scientific 1-Step Human High-Yield In Vitro Translation Kit to rapidly express highly functional caspase-3 enzyme, as well as how the system can be used to screen modulators of protein activity.

The 1-Step Human High-Yield IVT Kit is based on HeLa cell extracts that contain the necessary mammalian cellular machinery for protein expression and folding. Krishna Vattem cloned the caspase-3 gene into pT7CFE1-CHis expression vector and expressed the protein using the 1-Step Human High-Yield Mini IVT Kit. He observed a 3-fold higher activity for caspase-3 expressed by human IVT compared to a recombinant protein expressed and purified from bacteria.


Read the Article:

Expression of highly active proteins using a cell-free human IVT system

Expression of highly active proteins using a cell-free human IVT system