Pierce Firefly Luciferase One-Step Glow Assay Kit

Glowing results in one step

By Douglas Hayworth

The new Pierce Firefly Luc One-Step Glow Assay Kit expands our growing line of luciferase assay reagents to provide a way to measure firefly luciferase activity in mammalian cell culture using a single reagent-addition step.

Several features make the kit especially suitable for luminometers without injectors and for high-throughput screening (HTS) applications. The convenient, one-step, homogenous protocol minimizes handling steps to support the use of automation. Simply combine the supplied substrate solution and assay buffer to make a single working solution, and then add it to microplate wells containing the transfected, treated cells. The stable signal output (glow) of this system provides flexibility with regard to incubation time.

Highlights:

  • Sensitive – highly sensitive detection of firefly luciferase activity
  • Convenient – one-step, mix-and-read protocol; no additional reagents required
  • Stable – increased signal half-life and stability compared to flash assays
  • Simple – does not require a luminometer with injectors
  • Automation-friendly – amenable to high throughput assays

View Products:

Pierce Firefly Luciferase One-Step Glow Assay Kit

Pierce Firefly Luciferase One-Step Glow Assay Kit

All Luciferase Reporter Assays

All Luciferase Reporter Assays