New article on performance of spin desalting columns

Comparing desalting columns for protein recovery performance

By Douglas Hayworth

In a new product applications article, we compared small and large sizes of Thermo Scientific Zeba Spin Desalting Columns with closest equivalent columns from two other manufacturers for desalting performance with pure-protein and cell-lysate samples.

Several manufacturers offer spin desalting columns with 5K- to 7K-MWCO resins for rapid processing of 0.01mL to 4mL protein samples. Our team tested various columns for desalting performance with regard to efficiency of salt removal, protein recovery and sample-dilution effects. Overall, Zeba Columns provided the best protein recovery (with minimal or no sample dilution) for the widest range of sample volumes and concentrations tested.


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Benchmarking experiments with Zeba Spin Desalting Columns

Benchmarking experiments with Zeba Spin Desalting Columns