2014 Articles, Previews Volume 18

2014 Articles

Product research experiments and application data reports for the year 2014.

Product articles are research-style reports of experiments that demonstrate methods, specification claims and applications for new and existing Thermo Scientific Pierce Protein Biology reagents, kits and services. 2014 is the third full year of web-only publication of our former print newsletter called “Previews”.  The online format provides immediate access to featured product pages and the opportunity for you to interact with article authors via comments.

Volume Contents:

EMSA gel-to-nylon transfer with a fast semi-dry blotter

Transfer EMSA gels using the Pierce G2 Fast Blotter

The Pierce G2 Fast Blotter provides rapid semi-dry transfer of DNA (TBE) gels to nylon membrane

May 22, 2014

MEF primary cell isolation

An improved method for highly efficient isolation of primary mouse embryonic fibroblasts

Easily isolate and culture primary mouse embryonic fibroblasts (MEF)

May 19, 2014

New quantitative IP assay system

Pierce qIP Assay: a novel, HTS-compatible protein-protein interaction assay system

A cell-based, quantitative co-immunoprecipitation method for protein interaction analysis involving a sensitive luciferase assay strategy

May 2, 2014

Isolate primary cardiomyocytes

One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Easily isolate and culture highly viable, functional primary cardiomyocytes

April 2, 2014

Glycoprotein expression with human IVT system

Glycoprotein expression in a human IVT system

Expression of partially N-linked glycoproteins using the 1-Step Human Coupled in vitro Translation System

March 24, 2014

Desalting column comparison

Benchmarking experiments with Zeba Spin Desalting Columns

Protein recovery performance comparisons of Thermo Scientific Zeba Spin Desalting Columns to other commercially available desalting resins and columns

March 24, 2014

Number of proteins per agarose bead

Calculate the number of immobilized proteins per bead of agarose affinity supports

A consideration of relative sizes and dimensions of agarose resin beads, antibodies, proteins, chemical modification groups, and affinity ligands

March 3, 2014

Improved B-PER Bacterial Protein Extraction Reagent

Improved, all-in-one B-PER Reagent for bacterial protein extraction

B-PER Complete Reagent provides gentle, efficient, and cost effective protein extraction from E. coli and other bacterial cells

February 19, 2014

Method for primary neuron isolation

An efficient method for the isolation of highly functional primary neurons

Easily and reproducibly isolate and culture primary neurons

January 28, 2014

Express active caspase-3 using human IVT system

Expression of highly active proteins using a cell-free human IVT system

Active caspase-3 produced by cell-free protein expression with the 1-Step Human High-Yield In Vitro Translation Kit is more functional than recombinant caspase-3 expressed in bacteria

January 16, 2014

HTP high-yield IVT protein expression

System for high-throughput, high-yield in vitro translation

HTP protein expression using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis Plates

January 10, 2014