1-Step Ultra TMB-Blotting Solution

Ultra-colorimetric Western blot detection

By Douglas Hayworth

The new 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

This precipitating, colorimetric substrate for HRP provides high sensitivity, increased signal-to-noise ratio and low background compared to many other chromogenic substrates. The blotting solution contains soluble TMB (3,3′,5,5′-tetramethylbenzidine), which reacts very quickly with horseradish peroxidase enzyme to produce an insoluble dark blue precipitate that does not fade or flake. The substrate is compatible with both nitrocellulose and PVDF membranes. The blotting solution is supplied ready to use with no mixing required.


  • Fast – protein bands visible in less than one minute
  • Fade-resistant – protein bands stable after membrane drying
  • Sensitive – detection limit similar to standard ECL substrates
  • Chromogenic – no special equipment needed for visualization; produces dark blue bands
  • Ready to use – no organic solvents are required to dissolve; no dilution necessary for use

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1-Step Ultra TMB-Blotting Solution

1-Step Ultra TMB-Blotting Solution

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