New article demonstrates magnetic chromatin IP kit

Powerful ChIP-based analysis of androgen receptor biology

By Douglas Hayworth

A new product article describes results of our scientists’ recent study of factors affecting transcriptional regulation by the androgen receptor (AR) and demonstrates one powerful application for the Thermo Scientific Pierce Magnetic ChIP Kit.

Smith et al. examined the effects of testosterone, β-estradiol, and EGF stimulation on the androgen-sensitive prostate cancer cell line LNcaP. They used Western blot analysis to show activation/repression of proliferation pathways along with AR protein levels. They then investigated via chromatin immunoprecipitation (ChIP) assay whether or not non-androgen stimuli (β-estradiol and EGF) could lead to AR binding to the known androgen receptor elements (AREs) of several genes: prostate specific antigen (PSA), FK506 binding protein 5 (FKBP5), insulin-like growth factor 1 (IGF-1), cyclin-dependent kinase inhibitor 1A (CDKN1A), and transmembrane protease, serine 2 (TMPRSS2).


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Analysis of androgen-dependent and -independent regulation of transcriptional activity

Analysis of androgen-dependent and -independent regulation of transcriptional activity