New article on IVT with mRNA templates

Got mRNA? Express it by in vitro translation

By Douglas Hayworth

A new product article about cell-free protein expression describes a protocol for in vitro protein synthesis from mRNA templates using components of the Thermo Scientific 1-Step Human Coupled IVT Kit (primarily for DNA templates).

Our researchers used a commercially available in vitro transcription kit to produce turboGFP mRNA. Then they optimized and tested the translation protocol using this control system. The article includes example results, as well as tips for easily evaluating the protocol based on fluorescence of the GFP protein that is expressed by translation from the tGFP mRNA template.


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Protocol for in vitro protein expression using mRNA templates

Protocol for in vitro protein expression using mRNA templates