alamarBlue Cell Viability Reagent

Measure cell viability by fluorescence or absorbance

By Douglas Hayworth

Thermo Scientific alamarBlue Cell Viability Assay Reagent is a growth indicator to quantitatively measure cell viability and proliferation using fluorescent or colorimetric detection strategies.

The alamarBlue Reagent is a non-toxic, water-soluble resazurin dye (a redox indicator) that yields a fluorescent signal and a colorimetric change when incubated with metabolically active cells. The reagent is designed for cell viability and proliferation studies using mammalian cell lines, bacteria and fungi and is ideally suited for in vitro cytotoxicity studies. Additionally, cell viability can be monitored in real time when performing a variety of tissue engineering applications.


  • High sensitivity and linearity – detect as few as 50 cells
  • Quantitative – accurate measurement over time
  • Non-toxic to cells – low interference with metabolism
  • Scalable – a mix-and-read, homogeneous assay
  • No cell lysis – use with time-course or functional assays
  • Stable – monitor continuous cell growth
  • Flexible – cultured cells, bacteria and fungi
  • Versatile – fluorescent or colorimetric detection

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alamarBlue Cell Viability Assay Reagent

alamarBlue Cell Viability Assay Reagent