Investigate RNA-Protein interactions
A magnetic-bead kit to label RNA and enrich RBPs.
Use the new Pierce Magnetic RNA-Protein Pull-Down Kit to end-label RNA with desthiobiotin and then enrich for target RNA-binding proteins. The complete kit contains sufficient reagents for 20 RNA labeling reactions and 20 protein-RNA pull-down assays.
Direct enrichment protein-RNA interactions via labeled RNA provides an alternative to antibody capture of protein-RNA complexes or moieties incorporated into the nucleic acid. An added advantage to the kit is that it includes validated controls for both the labeling and pull-down assay. The kit is amenable to several downstream applications, including Western blotting and Mass Spectrometry (MS).
Highlights:
- Direct – capture ribonucleoprotein complexes with end-labeled RNA
- Easy to use – no spin cups or centrifugation necessary for the enrichment
- Flexible – use in vitro transcribed RNA or synthetic RNA for labeling
- Specific – low bead background; unrelated RNA does not interfere
- Economical – less expensive than synthesized end-labeled RNA, magnetic beads, etc.
- Complete – contains both labeling and enrichment modules
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