New article on dual-emission luciferase assays

New article on dual-spectral luciferase assays

By Douglas Hayworth

In this new product article, our researchers demonstrate how they performed experiments to confirm the efficacy of Thermo Scientific Pierce Luciferase Dual Assay Kits to spectrally resolve and quantify the activity of two luciferases using a commercial plate reader capable of simultaneous measurement at two different wavelengths. Pierce Luciferase Dual Assays deliver high sensitivity in a simple one-step detection protocol. They use pairs of luciferase enzymes that are luminesce at different wavelengths, allowing their activities to be distinguished and measured in the same samples. In this case, the authors validated an instrument that has detection mode whereby two photomultiplier tubes (PMT) enable the light from both luciferase reporters to be measured simultaneously with filters.


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Simultaneous dual-emission detection of luciferase reporter assays

Simultaneous dual-emission detection of luciferase reporter assays