New article demonstrates a triple luciferase assay method

Dual assays? Try triple luciferase assays!

By Douglas Hayworth

A new product article about luciferase assay strategies describes how our researchers developed and demonstrated a method to simultaneously assay the expression of two different genes relative to a third (normalizing) gene.

The triple luciferase reporter assay system uses Thermo Scientific Pierce Luciferase Assay Vectors and Kits (Gaussia, Cypridina, and Red Firefly luciferases). Choi et al. used the system with neuroblastoma cells (IMR-32) to test whether or not the parkinsonian neurotoxins paraquat, rotenone and MPP+ can activate ARE- and NF-κB-dependent transcription normalized by CMV-driven transcription. In so doing, the researchers demonstrate the practical application of this triple luciferase assay strategy to simultaneously monitor two pathways while normalizing to a third reporter of baseline activity.


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