New article about mass spectrometry sample preparation

Comparison of proteomic sample prep protocols for mass spec

By Douglas Hayworth

A new product article about mass spectrometry sample preparation methods compares four procedures for cell lysis, reduction, alkylation, digestion and peptide clean-up. Our scientists describe how they developed an optimized protocol and kit of reagents that standardize peptide sample preparation for MS analysis.

The protocol reproducibly yields high-quality peptide samples for LC-MS/MS analysis that provide high rates of protein identification. The method yields more protein lysate, is highly reproducible, is scalable from 10µg to 5mg, and results in higher protein identification rates than other popular “standard” sample preparation methods. The kit also includes a unique, non-mammalian internal digestion control standard protein (Digestion Indicator) to assure protocol performance and to quantify sample preparation processing and digestion efficiency across samples.


Read the Article:

A versatile mass spectrometry sample preparation procedure for complex protein samples

A versatile mass spectrometry sample preparation procedure for complex protein samples