2013 Articles, Previews Volume 17

2013 Articles

Product research experiments and application data reports for the year 2013.

Product articles are research-style reports of experiments that demonstrate methods, specification claims and applications for new and existing Thermo Scientific Pierce Protein Biology reagents, kits and services. 2013 is the second full year of web-only publication of our former print newsletter called “Previews”.  The online format provides immediate access to featured product pages and the opportunity for you to interact with article authors via comments.


Volume Contents:

Synergy Plate Readers and Thermo Scientific Luciferase Assays

Luciferase assays and BioTek Synergy microplate readers

Validation and comparison of single-step flash and dual-spectral luciferase reporter gene assays using the Synergy line of microplate readers

November 12, 2013

Webinar: Protein-Nucleic Acid Interactions

Strategies for the isolation and detection of protein-nucleic acid interactions

Webinar

October 25, 2013

IVT vectors and purification strategies

Choosing a vector and purification method for in vitro protein expression

Strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientific 1-Step High Yield IVT protein expression system

October 22, 2013

Experiments using magnetic ChIP (chromatin IP)

Analysis of androgen-dependent and -independent regulation of transcriptional activity

Monitor activity using a sensitive and optimized magnetic ChIP kit

September 11, 2013

Membrane Protein Extraction

Efficient mammalian membrane protein extraction

Fast and simple enrichment of integral membrane proteins and membrane-associated proteins

August 29, 2013

Tandem mass spectrometry and S-nitrosylation

Specific labeling, enrichment and quantitation of S-nitrosylated peptides using iodoTMT reagents

Measure protein and peptide cysteine modifications using multiplex quantitative mass spectrometry

August 19, 2013

Performance of Pierce Glutathione Superflow Agarose

Performance characterization of Pierce Glutathione Superflow Agarose

Purity, dynamic binding capacity, reusability and chemical compatibility properties of a glutathione resin for FPLC purification of GST tagged fusion proteins

August 2, 2013

Performance of HisPur Ni-NTA Superflow Agarose

Performance characterization of HisPur Ni-NTA Superflow Agarose

Purity, dynamic binding capacity, reusability and chemical compatibility properties of a nickel-charged IMAC resin for FPLC purification of His-tagged proteins

July 11, 2013

Western blot ECL imaging: X-ray film versus CCD camera

Improved Western blot detection using CCD digital imaging

Comparison of cooled-CCD digital imaging versus X-ray film for sensitivity, dynamic range and signal linearity with enhanced chemiluminescence (ECL)

June 26, 2013

Microplate luminometers for flash-type luciferase assays

Versatile luciferases: new tools for reporter assays

Microplate luminometers equipped with reagent dispensers and optical filters provide optimal performance for luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase reporter genes

June 24, 2013

Immunogen carrier protein activation and conjugation data

Carrier protein activation and conjugation data for immunogen preparation

Experiments with KLH variants and other immunogens to validate solubility, maleimide activation consistency, peptide conjugation efficiency and immunogenicity

June 5, 2013

Mass spectrometry sample preparation procedure for protein samples

A versatile mass spectrometry sample preparation procedure for complex protein samples

Prepare high-quality peptide digests with high recovery from cultured cells

June 4, 2013

Protocol for in vitro protein expression using mRNA templates

Protocol for in vitro protein expression using mRNA templates

Use the 1-Step Human Coupled DNA IVT kit to efficiently translate mRNA templates

May 22, 2013

Performance of HisPur Cobalt Superflow Agarose

Performance characterization of HisPur Cobalt Superflow Agarose

Purity, dynamic binding capacity, reusability and chemical compatibility properties of a cobalt-charged IMAC resin

May 2, 2013

Luciferase assays of pesticide chemical effects

Activation of the antioxidant response pathway by pesticide chemicals

Simultaneous measurement of the activation of ARE- and NF-κB-driven luciferase reporters by parkinsonian neurotoxins in neuronal cells

April 30, 2013

Separation characteristics of dialysis membranes

Separation characteristics of dialysis membranes

Molecular weight cut-off (MWCO) specifications and rates of buffer exchange with Slide-A-Lyzer Dialysis Devices and Snakeskin Dialysis Tubing

April 18, 2013

Monitor neuronal differentiation using luciferase assays

Monitoring neuronal differentiation using multiplexed luciferase reporters

Characterization of early neuronal differentiation markers in precursor cell lines using multiplexed luciferase reporters and immunofluorescence imaging

March 8, 2013

Chromatography cartridge performance data

Chromatography cartridges for desalting and affinity purification

Data using the cartridge format for automated liquid chromatography (LC) systems

March 7, 2013

Simultaneous dual-emission detection of luciferase assays

Simultaneous dual-emission detection of luciferase reporter assays

Performing dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate reader

January 24, 2013