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Obtain strong luciferase signals in cells with low transfection efficiencyAlthough plasmid-based luciferase assays are powerful tools for studying signal transduction pathways, cells with low transfection efficiency can make signal detection difficult. In this Product Article about performing luciferase assays in Jurkat cells, our scientists demonstrate the effectiveness of using the Thermo Scientific Pierce Luciferase Reporter Systems to transfect Jurkat cells and the Thermo Scientific Pierce Luciferase Flash Assay Kits to measure activity. Our luciferase assay systems produce a robust bioluminescent signal, enabling luciferase-based pathway studies in cells that are difficult to transfect.
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