Product Article on luciferase assays in Jurkat cells

Obtain strong luciferase signals in cells with low transfection efficiency

By Douglas Hayworth

Although plasmid-based luciferase assays are powerful tools for studying signal transduction pathways, cells with low transfection efficiency can make signal detection difficult. In this Product Article about performing luciferase assays in Jurkat cells, our scientists demonstrate the effectiveness of using the Thermo Scientific Pierce Luciferase Reporter Systems to transfect Jurkat cells and the Thermo Scientific Pierce Luciferase Flash Assay Kits to measure activity. Our luciferase assay systems produce a robust bioluminescent signal, enabling luciferase-based pathway studies in cells that are difficult to transfect.

 


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Luciferase assays in hard-to-transfect Jurkat cells

Luciferase assays in hard-to-transfect Jurkat cells