Obtain neuronal proteins from brain tissue and primary cells
By Douglas Hayworth
Neurons have unique morphology and lipid content, making protein extraction from this cell type very challenging. In this Product Article about neuronal protein extraction, our scientists show how the Thermo Scientific N-PER Neuronal Protein Extraction Reagent enables total protein isolation from neuronal tissue or primary cultured cells without adversely affecting protein function. Protein extraction was evaluated by quantifying total protein yield and analyzing proteins from different neuronal compartments by Western blot. Reagent compatibility with several downstream activity assays related to neuronal protein function was also tested.
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