Luciferase reporter assays for studying cell signal pathways
By Douglas Hayworth
Reporter gene assays are used to study complex cellular signaling pathways. In this Product Article about luciferase assays, our scientists demonstrate how dual-spectral luciferase assay kits can be used to monitor NFkB and CRE promoter activity. Using Cypridina/red firefly spectral separation multiplexing and Gaussia/Cypridina multiplex assays, our scientists were able to perform real-time monitoring of reporter activity in the media and simultaneous detection of spectrally resolvable luciferases using filter-based detection.
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