Product Article on subcellular protein fractionation

Isolate subcellular proteins from tissue samples

By Douglas Hayworth

The study of protein localization and translocation is important for understanding protein functions and diseases, especially cancers. Subcellular protein fractionation enables protein localization discoveries, enhances detection of low-abundance species, reduces sample complexity and enables the monitoring of physiologic fluxes and redistribution under basal and stimulated/diseased conditions. Tissue samples, however, present an extra level of complexity with their tightly woven network of proteins and extracellular matrices.

In this Product Article about obtaining subcellular proteins, our scientists demonstrate the effectiveness of the Thermo Scientific Subcellular Protein Fractionation Kit for Tissues. The kit was validated using a variety of mouse tissue samples. Protein localization was assessed for each tissue using ubiquitous, abundant or specific protein markers for each tissue type. Furthermore, proteins were assayed for functional activity in downstream assays.

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Fractionate subcellular proteins from tissue samples

Fractionate subcellular proteins from tissue samples