2012 Articles, Previews Volume 16

2012 Articles

Product research experiments and application data reports for the year 2012.

Product articles are research-style reports of experiments that demonstrate methods, specification claims and applications for new and existing Thermo Scientific Pierce Protein Biology reagents, kits and services. This is the first full year of web-only publication of our former print newsletter called “Previews”.  The new online format provides immediate access to featured product pages and the opportunity for you to interact with article authors via comments.

Volume Contents:

Quantitative phosphoprotein profiling

Quantitative cancer stem cell phosphoprotein profiling

Using tandem mass labeling and LC-MS/MS to profile perturbations of an autocrine loop

December 11, 2012

Phosphoprotein Enrichment

Phosphoprotein enrichment from cell and tissue samples

Isolate phosphorylated proteins with an easy-to-use spin format

November 12, 2012

Protein standards for quantitative mass spectrometry

Cell-free protein expression system for generating stable isotope-labeled proteins

Rapidly express protein standards for quantitative mass spectrometry

October 11, 2012

Removing endotoxins from various sample types

Eliminate endotoxins from protein and antibody samples

A highly specific affinity resin enables endotoxin removal from various protein sources, sizes and charges

July 18, 2012

Removing endotoxins using a spin-column format

Removing endotoxins using a spin-column format

Fast and effective removal of endotoxins from protein samples

July 9, 2012

Luciferase assays

Luciferase assays in hard-to-transfect Jurkat cells

Obtain strong signals in cells with low transfection efficiency

June 13, 2012

Subcellular protein fractionation from tissues

Fractionate subcellular proteins from tissue samples

Obtain proteins from five cellular components using a tissue-optimized kit

May 18, 2012

Enrich neuronal synaptic proteins while maintaining phosphoprotein integrity

Method to isolate functional synaptosomes

Enrich neuronal synaptic proteins while maintaining phosphoprotein integrity

May 10, 2012

Inhibitor tablet protect proteins during lysis and extraction

Complete protein protection from proteases and phosphatases

Protease, phosphatase and combination inhibitor tablets provide a convenient format to protect proteins from degradation and dephosphorylation

April 18, 2012

Extract neuronal proteins with N-PER Neuronal Protein Extraction Reagent

Optimized protein extraction reagent for neuronal tissue and primary cells

Obtain high yields of functional neuronal protein from all neuronal cell compartments

March 5, 2012

Luciferase reporter assays

Highly sensitive multiplex luciferase reporter assays

Monitor changes in NFkB and CRE promoter activity using bioluminescent reporter assays

February 28, 2012

Antibody-binding protein column formats

Using antibody-binding proteins for antibody purification

Choose a format based on the specific application and scale

February 24, 2012