Thermo Fisher Scientific Offers New Reagents to Study Kinases

By Kevin

 

FOR IMMEDIATE RELEASE
Media Contact Information:
Name: Monica O’Hara
Phone: +1 815-968-0747
Email: monica.ohara@thermofisher.com
Website: www.thermoscientific.com/pierce

 

ROCKFORD, Ill. (Jan. 25, 2011) – Thermo Fisher Scientific, the world leader in serving science, today introduced the Thermo Scientific Pierce Kinase Enrichment Kits. These kits use novel ATP- and ADP-based probes developed by AcitvX Biosciences to covalently modify active sites of ATPases, including kinases, chaperones and metabolic enzymes.

The probes feature a desthiobiotin tag, a biotin analog, attached to the nucleotide. The nucleotide binds to the active site of the kinase, whereas the desthiobiotin tag allows for capture and enrichment of the bound kinase. The desthiobiotin tag binds less tightly to biotin-binding proteins, making the capture bond easily reversible by biotin displacement, low pH or heat. The isolated proteins then can be further studied by Western blot analysis or mass spectrometry. The system is useful for inhibitor profiling and IC50 determinations.

The Pierce* Kinase Enrichment Kit is available with either an ActivX ATP- or ADP-based probe and includes all labeling and enrichment reagents. Both ActivX* Probes are also available separately.

For more details on the Pierce Kinase Enrichment Kit, please visit www.thermoscientific.com/pierce.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of more than $10 billion, we have approximately 35,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through two premier brands, Thermo Scientific and Fisher Scientific, which offer a unique combination of continuous technology development and the most convenient purchasing options. Our products and services help accelerate the pace of scientific discovery, and solve analytical challenges ranging from complex research to routine testing to field applications. Visit www.thermofisher.com.


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Kinase Enrichment Kits and Probes

Kinase Enrichment Kits and Probes