Spectral properties of Thermo Scientific DyLight Fluors.
DyLight Dyes have absorption maxima ranging from 350nm to 770nm. The emission profiles correspond to the principal output wavelengths (filter sets and lasers) of standard fluorescence imagers, making them compatible with fluorescence microscopy, fluorescence plate readers, array scanners and instrumentation such as the Typhoon* and LI-COR Odyssey* Imagers.
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DyLight Fluor Product Guide
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| Spectral Properties of DyLight Fluorescent Dyes |
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Emission
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Fluor
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Ex/Em†
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Spectra
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e††
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Spectrally Similar Dyes
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Blue
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353/432
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15K
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Alexa Fluor* 350, AMCA
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Blue
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400/420
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30K
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Alexa Fluor 405, Cascade Blue*
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Green
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DyLight 488
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493/518
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70K
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Alexa Fluor 488, fluorescein, FITC
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Yellow
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DyLight 549
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562/576
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150K
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Alexa Fluor 546, Alexa Fluor 555,
Cy3*,TRITC
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Red
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DyLight 594
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593/618
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80K
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Alexa Fluor 594, Texas Red*
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Red
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DyLight 633
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638/658
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170K
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Alexa Fluor 633
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Red
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DyLight 649
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654/673
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250K
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Alexa Fluor 647, Cy5*
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Near IR
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DyLight 680
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692/712
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140K
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Alexa Fluor 680, Cy5.5*
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Near IR
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DyLight 750
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752/778
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210K
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Alexa Fluor 750
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Infrared
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DyLight 800
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777/794
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270K
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IRDye* 800
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†Excitation and emission maxima in nanometers (+/- 4nm) in phosphate-buffered saline (PBS)
††Molar extinction coefficient (M-1 cm-1) at the absorption maximum |
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Overlay of all
DyLight Fluor
emission spectra
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DyLight 350 Dye
Absorption (black)
Emission (blue)
Replaces:
– Alexa Fluor 350 Dye
– AMCA Dye
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DyLight 405 Dye
Absorption (black)
Emission (blue)
Replaces:
– Alexa Fluor 405 Dye
– Cascade Blue* Dye
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DyLight 488 Dye
Absorption (black)
Emission (green)
Replaces:
– Alexa Fluor 488 Dye
– Fluorescein
– FITC
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DyLight 549 Dye
Absorption (black)
Emission (yellow)
Replaces:
– Alexa Fluor 546 Dye
– Alexa Fluor 555 Dye
– Cy3
– Rhodamine
– TRITC
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DyLight 594 Dye
Absorption (black)
Emission (orange-red)
Replaces:
– Alexa Fluor 594 Dye
– Texas Red* Dye
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DyLight 633 Dye
Absorption (black)
Emission (red)
Replaces:
– Alexa Fluor 633 Dye
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DyLight 649 Dye
Absorption (black)
Emission (red)
Replaces:
– Alexa Fluor 647 Dye
– Cy5 Dye
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DyLight 680 Dye
Absorption (black)
Emission (brown, near IR)
Replaces:
– Alexa Fluor 680 Dye
– Cy5.5 Dye
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DyLight 750 Dye
Absorption (black)
Emission (brown, near IR)
Replaces:
– Alexa Fluor 750 Dye
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DyLight 800 Dye
Absorption (black)
Emission (brown, infrared)
Replaces:
– IRDye* 800 Dye
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* Trademark; see link in page footer.
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