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Western Blot Video Tutorials

Western blot analysis is one of the most common methods used in the lab today, because of its versatility in detecting and measuring specific target proteins in a sample and revealing their activity and any post-translational modifications. Western blotting is a multi-step process that can take 1-2 days to complete, depending on the methods used. Below are videos that detail the common steps to perform a Western blot, from separating proteins in a lysate to developing the blot.

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Protein Methods Library Home


Overview of Western Blot Analysis

Western blot lysates
 
Separate proteins in a sample by SDS-PAGE
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Protein Electrophoresis and Staining
Transfer proteins from a gel to PVDF or nitrocellulose membrane for Western blot
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Western Blot Transfer Methods
Block your PVDF or nitrocellulose membrane to reduce Western blot background signal
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Blocking Membranes
Use primary and HRP-labeled secondary antibodies to detect your proteins by Western blot
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Probing With Antibodies
Detect your protein by chemiluminescent Western blot
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Chemiluminescent Western Blot Detection
Developed Western blot
 
Strip your Western blot membrane to reprobe for another target
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Stripping and Reprobing Western Blots
Stripped and reprobed Western blot
 
Written and/or reviewed by Jared Snider, Ph.D.

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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