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Protein Methods Library
 

General Protein Methods Special Techniques

Protein Expression

 • Overview
 • Cell-free Expression
 • GST-tagged Proteins
 • His-tagged Proteins

Electrophoresis & Staining

 • Overview
 • Protein Gel Stains
 • MW Marker Selection Guide
 • Gel Stain Selection Guide

ELISA

 • Overview
 • ELISA Development Guide
 • Substrate Selection Guide

Affinity Purification

 • Overview
 • Antibody Purification
 • Avidin-Biotin Systems
 • Immunoprecipitation
 • Covalent Immobilization

Western Blotting

 • Overview • Videos
 • Transfer Methods
 • Blocking Buffers
 • Stripping and Reprobing
 • Chemiluminescent Blotting
 • Colorimetric Substrates
 • Fluorescent Detection

Protein Extraction

 • Overview
 • Cell Lysis Solutions
 • Cell Fractionation
 • Organelle Enrichment
 • Protease Inhibitors
 • Detergents

Buffer Exchange

 • Overview
 • Dialysis
 • Desalting

Protein Assays

 • Overview
 • Chemistry
 • Data Analysis
 • Selection Guide

Immunohistochemistry

 • Overview
 • IHC vs. ICC
 • Tissue Fixation
 • Paraffin Removal
 • Quenching
 • Blocking Strategies
 • Immunodetection
 • Avidin-Biotin for IHC
 • Immunofluorescence
 • IHC Counterstains
 • IHC Troubleshooting

Post-Translational Modifications

 • Overview
 • Phosphorylation
 • Glycosylation

Protein-Nucleic Acid Interactions

 • Overview
 • Protein-DNA Methods
 • Protein-RNA Methods
 • Chromatin IP (ChIP)
 • EMSA (Gel Shift Assays)
 • Labeling Nucleic Acids

Protein-Protein Interactions

 • Overview
 • Co-Immunoprecipitation (Co-IP)
 • Pull-down Assays
 • Crosslinking Analysis
 • Label Transfer Analysis
 • Far-Western Blot Analysis

Antibody Production

 • Overview
 • Immunogen Preparation
 • Antibody Screening-Isotyping
 • Antibody Purification
 • Antibody Fragmentation

Protein Detection Probes

 • Overview
 • Secondary Antibodies
 • Avidin-Biotin Probes
 • Enzyme Conjugates
 • Luciferase Reporters
 • Fluorescent Probes

Crosslinking

 • Overview
 • Chemistry
 • Applications
 • Selection Guide

Protein Modification

 • Overview
 • PEGylation
 • Protease Selection

Mass Spectrometry

 • Overview
 • Quantitative Proteomics
 • Sample Preparation
 • Workflow diagram

Peptides

 • Peptide Design
 • Peptide Synthesis

Protein Labeling

 • Overview
 • Biotinylation
 • Enzyme Conjugation
 • Fluorescent Labeling
 • Metabolic Labeling
 • DNA & RNA Labeling

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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