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Carbodiimide Crosslinker Chemistry

Carboxyl-reactive chemical groups in biomolecular probes for labeling and crosslinking carboxylic acids to primary amines include the carbodiimide compounds EDC and DCC. This article describes the reaction chemistries and biology research applications of this class of reagents.

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Protein Methods Library Home

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Chemistry of Crosslinking (summary of all reactive groups)

Overview of Crosslinking


Carboxyl-reactive Crosslinker Reactive Groups

Very few types of chemical groups are known to provide specific and practical conjugation to carboxylic acids (–COOH), such as occur in proteins and many other biomolecules. Certain diazomethane and diazoacetyl reagents have been used to derivatize small compounds for analysis by HPLC or for fluorescent labeling. Carbonyldiimidazole (CDI) can be used in non-aqueous conditions to activate carboxylic acids for direct conjugation to primary amines (–NH2) via amide bonds.

Carbodiimide compounds provide the most popular and versatile method for labeling or crosslinking to carboxylic acids. The most readily available and commonly used carbodiimides are the water-soluble EDC for aqueous crosslinking and the water-insoluble DCC for non-aqueous organic synthesis methods.

EDC carbodiimide crosslinker chemical structure DCC carbodiimide crosslinker chemical structure
Chemical structures of carbodiimides EDC and DCC. EDC (also called EDAC) is 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride, MW 191.70. DCC is N',N'-dicyclohexyl carbodiimide, MW 206.32.

Carbodiimides, as with CDI-mediated conjugation, work by activating carboxyl groups for direct reaction with primary amines via amide bond formation. Because no portion of their chemical structure becomes part of the final bond between conjugated molecules, carbodiimides are considered zero-length carboxyl-to-amine crosslinkers.

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Bioconjugate Techniques, Greg Hermanson
Bioconjugate Techniques,
2nd Edition (2008)
Greg T. Hermanson,
Academic Press, Inc.,
1202 pages.

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EDC (also called EDAC)
1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride


EDC Carbodiimide Crosslinker

EDC Reaction Chemistry

EDC reacts with carboxylic acid groups to form an active O-acylisourea intermediate that is easily displaced by nucleophilic attack from primary amino groups in the reaction mixture. The primary amine forms an amide bond with the original carboxyl group, and an EDC by-product is released as a soluble urea derivative. The O-acylisourea intermediate is unstable in aqueous solutions; failure to react with an amine results in hydrolysis of the intermediate, regeneration of the carboxyls, and the release of an N-unsubstituted urea.

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EDC carbodiimide crosslinker

Amine-Biotin Reagents
for labeling carboxyls with EDC


Carboxyl-to-amine crosslinker reaction scheme for the carbodiimide EDC
EDC (carbodiimide) crosslinking reaction scheme. Carboxyl-to-amine crosslinking with the popular carbodiimide, EDC. Molecules (1) and (2) can be peptides, proteins or any chemicals that have respective carboxylate and primary amine groups. When they are peptides or proteins, these molecules are tens-to-thousands of times larger than the crosslinker and conjugation arms diagrammed in the reaction.

EDC crosslinking is most efficient in acidic (pH 4.5) conditions and must be performed in buffers devoid of extraneous carboxyls and amines. MES buffer (4-morpholinoethanesulfonic acid) is a suitable carbodiimide reaction buffer. Phosphate buffers and neutral pH (up to 7.2) conditions are compatible with the reaction chemistry, albeit with lower efficiency; increasing the amount of EDC in a reaction solution can compensate for the reduced efficiency.

N-hydroxysuccinimide (NHS) or its water-soluble analog (Sulfo-NHS) is often included in EDC coupling protocols to improve efficiency or create dry-stable (amine-reactive) intermediates. EDC couples NHS to carboxyls, forming an NHS ester that is considerably more stable than the O-acylisourea intermediate while allowing for efficient conjugation to primary amines at physiologic pH.

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MES Buffered Saline
0.1 M MES, 0.9% NaCl, pH 4.7

NHS and Sulfo-NHS

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NHS Ester Crosslinker Chemistry

Carboxyl-to-amine crosslinker reaction scheme for EDC (carbodiimide) and Sulfo-NHS.
Sulfo-NHS plus EDC (carbodiimide) crosslinking reaction scheme. Carboxyl-to-amine crosslinking using the carbodiimide EDC and sulfo-NHS. Addition of NHS or Sulfo-NHS to EDC reactions (bottom-most pathway) increases efficiency and enables molecule (1) to be activated for storage and later use.

EDC is also capable of activating phosphate groups in the presence of imidazole for conjugation to primary amines. The method is sometimes used to modify, label, crosslink or immobilize oligonucleotides through their 5' phosphate groups.

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Tech Tip #30: Modify and label oligonucleotide 5' phosphate groups

Applications for EDC Crosslinking

The ability to crosslink primary amines to carboxylic acid groups using EDC is a powerful and versatile tool for crosslinking peptides and proteins, preparing biomolecular probes, and immobilizing macromolecules for use in numerous protein and cell biology detection and analysis methods.

Of course, peptides and proteins contain both primary amines and carboxylic acids (N- and C-termini, respectively, as well as in the side-chain of certain amino acids). Thus, EDC enables peptides and proteins to be easily conjugated to one another or to any compounds or solid surfaces that bear either carboxyl or amino groups.

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Crosslinking Applications

Immunogen Preparation

Covalent Immobilization Methods for Affinity Purification

1. Peptide conjugation to carrier proteins

Because peptides and proteins contain both carboxylates and amines, EDC-mediated crosslinking usually causes random polymerization of polypeptides. This outcome is desirable when preparing immunogens for use in antibody production because it allows peptide antigens to be polymerized and conjugated at high densities onto immunogenic carrier proteins, such as KLH or BSA.

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KLH and EDC Conjugation Kit

BSA and EDC Conjugation Kit

2. Label carboxyl groups with amine compounds

EDC provides the only method for labeling or crosslinking to carboxyl groups of peptides or proteins (i.e., the C-terminus and side chains of glutamic acid and aspartic acid). To accomplish this without also reacting to a significant number of primary amines on the peptide or protein, one must supply a large molar excess of the desired amine-containing molecule. For example, to biotinylate only the C-terminus of a peptide, one would combine the peptide with something like a 100-fold molar excess of an amine-containing biotin compound before adding the appropriate amount of EDC. Most of the amines encountered by EDC-activated carboxylic acid groups of the peptide would be those of the biotin compound, so peptide-to-biotin conjugation would predominate over peptide-to-peptide conjugation.

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Amine-Biotin Compounds
(for carboxyl biotinylation)

Amine-PEG Compounds
(for carboxyl pegylation)

3. Immobilize peptides for affinity purification

Proteins are typically immobilized or crosslinked via primary amines or sulfhydryl groups rather than through carboxylates. By contrast, peptides (and other small carboxyl-containing molecules) are often immobilized using EDC to polymerize and conjugate them to an amine-derivatized surface material or solid support such as agarose resin. For example, Thermo Scientific CarboxyLink Coupling Resin is 4% beaded agarose that has been modified with diaminodipropylamine (DADPA); it provides the necessary amines for conjugation of peptide carboxylates at the end of a long spacer arm. Peptides are frequently immobilized to agarose beads by this method for antigen-specific affinity purification of antibodies following immunization of animals with peptide-KLH conjugates.

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Covalent Immobilization Methods for Affinity Ligands

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CarboxyLink Coupling Resins and Peptide Immobilization Kit

4. Attach peptides to surface materials

Besides agarose beads, many other solid materials are used as platforms to immobilizing molecules for assay methods. Primary amines and carboxylic acids are the most common foundational surface-derivatives for solid particles such as magnetic beads or glass slides. Aminosilane compounds such as 3-aminopropyl-triethoxysilane (APTS or APS) are popular reagents for coating glass (borosilicate) surfaces with primary amines. EDC is then one important means for covalently attaching peptides or other compounds to the surface material.

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Aminopropyltriethoxysilane (APTS) aminosilane reagent

Carboxy-PEG-Amine Compounds

DCC Carbodiimide Crosslinker

DCC Reaction Chemistry and Applications

DCC (dicyclohexyl carbodiimide) crosslinks carboxylic acids to primary amines in the same manner as EDC (see reaction schemes above). However, because DCC is not aqueous-soluble, it is primarily used in manufacturing and organic synthesis applications rather than in the typical protein research biology lab. For example, most commercially available, ready-to-use NHS-ester crosslinkers and labeling reagents are manufactured using DCC. Because water is excluded, the resulting NHS ester can be prepared and stabilized as a dried powder without appreciable hydrolysis. DCC is also commonly used in commercial peptide synthesis operations.

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DCC (dicyclohexyl carbodiimide)

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NHS Ester Crosslinker Chemistry

Peptide Synthesis

Written and/or reviewed by Douglas Hayworth, Ph.D.

Instructions | MSDS | CofA
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