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PEPotec SRM Peptide Libraries

Develop mid- to high-throughput SRM and MRM assays with these application-specific peptide libraries.


PEPotec Immuno peptide libraries for epitope mapping and high-throughput screeningThermo Scientific PEPotec SRM Peptide Libraries provide fully synthetic, crude peptides customized for the development of mid- to high-throughput selected reaction monitoring (SRM) and multiple reaction monitoring (MRM) assays in quantitative mass spectrometry (MS) workflows.

The study of proteomes, sub-proteomes and protein pathways often requires quantitative MS analysis that depends on the identification and validation of SRM and MRM assays. PEPotec SRM peptide libraries offer great convenience and flexibility in the development of quantitative MS approaches while accelerating biological assays by reducing the setup time of MS experiments. These peptide libraries were developed as a result of our involvement in the SRMAtlas project, which seeks to map the entire human proteome.

The standard service supplies a suspension of at least 0.1mg of each crude peptide housed in individual tubes in a 96-well plate format with either arginine (R) or lysine (K) as the C-terminal amino acid. Three quality control grades are available, and optional services and peptide modifications are offered to give you the peptide libraries that fit your experimental needs.

Highlights:

  • Convenient – peptides are provided in individual 2-D barcoded tubes in 96-tube plates
  • Application-specific – grades and optional services help you get the right peptides for your assay
  • Easy to use – standard libraries are delivered suspended in 0.1% trifluoroacetic acid (TFA) in 50% (v/v) acetonitrile/water
  • Flexible – extensive list of available modifications

Includes:

  • Fully synthetic crude (as synthesized) peptides
  • Multiple grades of QC analysis and optional services and modifications
  • Provided in individual Thermo Scientific Matrix 96-tube plates

Applications:

  • Mid- to high-throughput development of SRM and MRM assays
  • MS workflows with relative and absolute quantitation strategies
PEPotec SRM Peptide Libraries – Three grades to fit your experimental needs
  Grade 1
Fast and Easy
Grade 2
Greater Analysis
Grade 3
Maximum Assurance
Quantity >100μg
Length1 6 to 25 amino acids
Purity Crude (as synthesized)
Formulation1 Suspended in 0.1% TFA in 50% (v/v) acetonitrile/water
Delivery format Thermo Scientific Matrix 96-tube plates (Part No. 3712MTX)
C-terminal residue1 R or K
Counter ion TFA
Quality control (QC) MS check of 5% of peptides MS check of 100% of peptides MS analysis of 100% of peptides
Peptide resynthesis2 Not provided Not provided One resynthesis provided
Failed synthesis policy You pay for entire set of peptides ordered You only pay for peptides synthesized You only pay for peptides synthesized
Included documentation Peptide amount Peptide amount Peptide amount and
MS spectra
Minimum order3 24 peptides 4 peptides 4 peptides
1 Changes to the standard length restrictions, formulation and C-terminal residues are available as optional services
2 Peptides not detected during MS analysis will be resynthesized (depending on the grade selected)
3 Orders below 48 peptides incur a plate fee

PEPotec SRM Peptide Library Optional Services
Modification/Service Unit Size
Peptides that are 3-5 or 26-35 amino acids in length Peptide
QC: Add analytical HPLC of 100% of peptides4 Plate
QC: LC-MS of 100% of samples
Peptide lyophilization
Individually labeled tubes
4 Service is limited to Grade 3 libraries. Customers will receive documentation for both MS and HPLC analysis of 100% of peptides.

PEPotec SRM Peptide Optional Modifications – Available with all grades on a per-peptide basis
Modification
Isotopic labeling at C-terminus with heavy R or K
Isotopic labeling at C-terminus with alternative heavy amino acid
Alternative light amino acid at C-terminus
Isotopic labeling at single internal site with heavy amino acid (A, R, I, L, K, F, P or V)
Phosphorylation at 1 site
Phosphorylation at 2 sites
Phosphorylation at 3 sites
All cysteines protected by carbamidomethylation (CAM)
Lysine acetylation at 1 site
Methionine sulfoxide
Methyl lysine at 1 site
Methyl lysine
Diglycine ubiquitination motif on lysine
3-chlorotyrosine
Methyl arginine
Isoaspartic acid
Formylation
Hydroxyproline

References:

ISB, ETH Zurich Complete Initial Draft of SRMAtlas Covering Entire Human Proteome. Bonislawski, A. GenomeWeb.com. Sept. 24, 2010.

Related Resources:

Peptide Analysis Tool
Antigen Profiler for Robust Peptide Antibody Design

Protein Methods Library: Peptide Synthesis
Protein Methods Library: Peptide Design

Related Products:

PEPotec Immuno Custom Peptide Libraries
Custom Peptide Synthesis Service
HeavyPeptide AQUA Custom Synthesis Service
HeavyPeptide IGNIS Prime Peptide Quantitation Kit

Learn about all Peptide Products


Quote Request and Ordering Information
Download the Quote Request/Order Form (1.7mb) Download the peptide order form

Submission Information
For North America:

Email quote requests or orders to
peps@thermofisher.com

OR

Contact your local sales representative or call 1-800-874-3723 or 1-815-968-0747.

For Europe and all other regions:

Email quote requests to services.biopolymers@thermofisher.com

Email orders to sales.peptides@thermo.com

Or contact your local sales representative or Thermo Fisher Scientific in Ulm, Germany at +49-731-29870.

For technical support, please call
1-800-874-3723 or 1-815-968-0747
For technical support, please call +49-731-29870.

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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