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Custom Expression Vector Construction Service


Custom cDNA vector construction for the Pierce Human In Vitro Protein Expression System.


Custom cDNA or ORF vector construction for the Pierce Human In Vitro Protein Expression System.

Custom expression vector construction services are available for inserting one or more cDNAs into an expression plasmid that is optimized for use with the Thermo Scientific Pierce Human In Vitro Protein Expression System.

To begin using the Pierce Human In Vitro Protein Expression System, your coding sequence must be flanked by the proper transcription and translation elements. The T7 Cell-Free Expression Vector (pT7CFE1-CHis) is a versatile cloning plasmid optimized for this purpose. It contains the critical EMCV IRES element needed to generate mRNA capable of producing high levels of cap-independent protein expression in eukaryotic expression systems.

Request Custom Synthesis:

Bio Basic Inc – our current preferred service provider for IVT vectors

Related Resources:

Getting Started Guide – overview of critical parameters for successful human in vitro protein expression

Related Products:

Pierce Human In Vitro Protein Expression Kits – optimized for high yield and general protein expression
pT7CFE1-CHis Human In Vitro Expression Vector – optimized for cap-independent protein expression


Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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