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Custom Expression Vector Construction Service


Custom cDNA vector construction for the Pierce Human In Vitro Protein Expression System.


Custom cDNA or ORF vector construction for the Pierce Human In Vitro Protein Expression System.

Custom expression vector construction services are available for inserting one or more cDNAs into an expression plasmid that is optimized for use with the Thermo Scientific Pierce Human In Vitro Protein Expression System.

To begin using the Pierce Human In Vitro Protein Expression System, your coding sequence must be flanked by the proper transcription and translation elements. The T7 Cell-Free Expression Vector (pT7CFE1-CHis) is a versatile cloning plasmid optimized for this purpose. It contains the critical EMCV IRES element needed to generate mRNA capable of producing high levels of cap-independent protein expression in eukaryotic expression systems.

Highlights:

  • Optimized – ensure your coding sequence is ready for optimal human in vitro protein expression
  • Economical – competitively priced for value and speed
  • Efficient – use our reliable service to help move from design to experimentation sooner

Service Options:

  • Option 1: Send a cDNA – we re-clone your sequence into our expression vector
  • Option 2: Buy a cDNA – we insert a cDNA from our library into our expression vector
  • Option 3: Synthesize a cDNA – we synthesize and clone your custom sequence into our expression vector

Custom Vector Cloning Services

Custom Vector Construction Service Options. Choose one or more of the custom services options described above. Complete and submit the Request Form. We will then contact you to verify the order, payment options as well as review shipping details.
Service Description of Custom Service Price per sequence
Option 1 Send a plasmid containing your coding sequence to us and we will insert it into pT7CFE1-CHis. $250
Option 2 Select a CloneID from our cDNA library and we will insert the coding sequence into pT7CFE1-CHis. $350
Option 3 Send us a coding sequence and we will synthesize the DNA and insert the fragment into pT7CFE1-CHis. $250 + $0.34/bp
† pricing includes $250 cloning service plus $0.34/base pair synthesis service

Related Resources:

Getting Started Guide – overview of critical parameters for successful human in vitro protein expression

Related Products:

Pierce Human In Vitro Protein Expression Kits – optimized for high yield and general protein expression
pT7CFE1-CHis Human In Vitro Expression Vector – optimized for cap-independent protein expression


Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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