Custom expression vector construction services are available for inserting one or more cDNAs into an expression plasmid that is optimized for use with the Thermo Scientific Pierce Human In Vitro Protein Expression System.
To begin using the Pierce Human In Vitro Protein Expression System, your coding sequence must be flanked by the proper transcription and translation elements. The T7 Cell-Free Expression Vector (pT7CFE1-CHis) is a versatile cloning plasmid optimized for this purpose. It contains the critical EMCV IRES element needed to generate mRNA capable of producing high levels of cap-independent protein expression in eukaryotic expression systems.
Request Custom Synthesis:
Bio Basic Inc – our current preferred service provider for IVT vectors
Getting Started Guide – overview of critical parameters for successful human in vitro protein expression
Pierce Human In Vitro Protein Expression Kits – optimized for high yield and general protein expression
pT7CFE1-CHis Human In Vitro Expression Vector – optimized for cap-independent protein expression