Western blotting is a method that remains a fundamental and integral part of research programs involving protein biology.
In this technique, protein samples derived from biological fluids or by lysis of cultured cells are first separated by gel electrophoresis and transferred onto PVDF or nitrocellulose membrane. Then proteins of interest are probed with specific antibodies that are tagged (most commonly with HRP enzyme), allowing those protein bands on the blot to be detected and imaged after tag-specific substrates are applied.
The Thermo Scientific Pierce product line includes reagents, kits, gels, membranes, antibodies, and equipment to facilitate every step of Western blot analysis.
Overview of Western Blotting
Western Blot Video Tutorials
Western Blot Reagents, Kits and Equipment