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SuperSignal ELISA Pico Substrate FAQ


Answers to frequently asked questions (FAQs) about SuperSignal ELISA Pico Chemiluminescent Substrate


 

At what wavelength is SuperSignal ELISA Pico Substrate signal measured?
The light emission wavelength is read at 425 nm.

Can I dilute the working reagent if my signal is too intense?
We do not recommend diluting the working reagent. It is better to dilute or otherwise optimize primary and or secondary antibody concentrations or use more washes between incubation steps.

How does this reagent compare with QuantaBlu Fluorogenic Peroxidase Substrate?
QuantaBlu Fluorogenic Peroxidase Substrate is a fluorescent detection reagent for HRP and provides attamole sensitivity. SuperSignal ELISA Pico Substrate is strictly chemiluminescent.

How sensitive is SuperSignal ELISA Pico Chemiluminescent Substrate?
The substrate allows measurement of low picogram levels in sandwich ELISA procedures.

What signal duration occurs with SuperSignal ELISA Pico Substrate?
Maximum signal is reached within five minutes and then declines slowly thereafter until it is much less after an hour.

What microplates work best with this substrate?
White plates provide the best signal-to-noise ratio. Black plates provide lower background but less detectable signal.

What type of instrument do I use to read the plates detected with this chemiluminescent substrate?
A luminometer is used to measure relative light units.

 

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