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SuperSignal ELISA Femto Substrate FAQ

Answers to frequently asked questions (FAQs) about SuperSignal ELISA Femto Maximum Sensitivity Chemiluminescent Substrate


At what wavelength is SuperSignal ELISA Femto Substrate measured?
The light emission wavelength is measured at 425 nm.

Can I dilute the working reagent if my signal is too intense?
We do not recommend diluting the working reagent. It is better to dilute or otherwise optimize primary and or secondary antibody concentrations or use more washes between incubation steps.

How sensitive is SuperSignal ELISA Femto Substrate?
Our researchers have detected down to 168 fg of interleukin-2 in sandwich ELISA.

What signal duration occurs with SuperSignal ELISA Femto Substrate?
Maximum signal is reached within two minutes and then declines steadily until it is neglible after twenty minutes.

What microplates work best with this substrate?
White plates provide the best signal-to-noise ratio. Black plates provide lower background but less detectable signal.

What type of instrument do I use to read the plates detected with this chemiluminescent substrate?
A luminometer is used to measure relative light units.


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