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SuperBlock Blocking Buffers FAQ


Answers to frequently asked questions (FAQs) about SuperBlock Blocking Buffers


 

Can SuperBlock Blocking Buffer be used with Western Blotting and ELISAs?
Yes, it can be used with both procedures, including those that use chemiluminescent detection.

Can plates be blocked with SuperBlock Blocking Buffer and then stored dry?
After the plates are blocked, remove the blocking buffer and air dry the plates for several hours. Store plates with desiccant in a plastic bag at 4°C. Change desiccant after 24 hours for optimal storage. The plates should be stable for at least 12 months.

Does SuperBlock Blocking Buffer contain albumin, biotin, DNA, detergent or phosphoproteins?
No.

Does SuperBlock Blocking Buffer need to be diluted?
No. It is supplied at 1X concentration that should be used "as is."

Does SuperBlock Blocking Buffer work with nylon and nitrocellulose membrane?
Yes.

Is SuperBlock Blocking Buffer RNase-free?
No.

What incubation time and temperature should I use for effective blocking?
Typical procedures use 1 hour at room temperature or overnight at 4°C. However, sufficient blocking in many procedures is possible in 3 x 2 minutes at room temperature.

What is SuperBlock Blocking Buffer's shelf life?
Its shelf life is one year at 4°C.

What preservative is used in SuperBlock Blocking Buffer, and what is its source?
Kathon* Preservative, a broad spectrum antimicrobial agent, is used at a concentration of 600 ppm.

What should I do about the colloidal carbon (black precipitate) in the solution?
Allow any colloidal carbon, if present, to it settle to the bottom of the bottle and pouring off what you need to use. We do not recommend centrifugation or filtering because these procedures may reduce the product's blocking capabilities.

 

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