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Slide-A-Lyzer MINI Dialysis Units FAQ


Answers to frequently asked questions (FAQs) about Slide-A-Lyzer MINI Dialysis Units


 

Does it leak?
The innovative, patented design does not permit "wicking" that can occur with homemade microdialysis devices.

How can I use the Slide-A-Lyzer MINI Dialysis Unit in my laboratory?
The Slide-A-Lyzer MINI Dialysis Unit is ideal for:

  • Salt reduction Buffer exchange (<10 min for pH change 2.8 to 9.5
  • Removal of small molecular weight compounds
  • Micro-conjugation (reagent addition or sample evaluation anytime)
  • Equilibrium dialysis (microtube, paraffin to adjust depth)
  • Concentration (in a microtube concentrates @ 35-45 µl/hr; use at least a 3:1 v/v ratio of concentrator to sample.)
  • Sample preparation for instrumentation analysis (HPLC, CE, MS, GC, electrophoresis, etc.)
  • Sample preparation for proteins, peptides, oligonucleotides, DNA, RNA (~330 MW/base ~660 MW/bp)
  • The "scavenger" technique in which a resin, membrane, polymer or protein is placed in a microtube with buffer in order to select a compound to remove from a sample in a Slide-A-Lyzer MINI Dialysis Unit.

How do I obtain optimum performance with the Slide-A-Lyzer MINI Dialysis Unit?
Always wear gloves when touching a Slide-A-Lyzer MINI Dialysis Unit If desired, soak the Slide-A-Lyzer MINI Dialysis Unit to remove contaminants For best recovery, tilt the tube and collect the sample in the bottom corner of the device

How do I remove trace contaminants?
To remove glycerol, dialyze for 15 minutes against 1 liter of deionized water. Glycerol contents in <3% in the 3K unit, ~15% in the 7K unit and 23% in the 10K unit. To remove metals, dialyze for 15 minutes against 1 liter of 1 mM EDTA. Metals present in the 3K, 7K and 10K unit include 2 ppb iron, 5 ppb magnesium, 1.5 ppb nickel, 0.2 ppb zinc, 0.2 ppb copper, 0.5 ppb chromium and 0.3 ppb cadmium. To remove sulfur, dialyze for 15 minutes against 1 liter of deionized water. Sulfur content should be minimal, <0.1%. Pierce quality control checks for sulfur on the membrane prior to the unit's assembly.

How do I use the Slide-A-Lyzer MINI Dialysis Unit?
Wearing gloves, apply sample (0.01-0.1 ml) using a pipette. OPTIONAL: Soak the MINI (see question below) to remove trace contaminants. Cap the Slide-A-Lyzer MINI Dialysis Unit (to minimize loss) and snap it into the float. Insert the float (sold separately) into the beaker containing the dialysate. Recover sample.

How long does it take to dialyze with the Slide-A-Lyzer MINI Dialysis Unit?
The Slide-A-Lyzer MINI Dialysis Unit can convert 100 µl of pH 2.8 buffer to pH 9.4 dialyzing against 1 liter bicarbonate buffer, pH 9.4 in less than 10 minutes.

What is the volume-to-membrane surface area for the Slide-A-Lyzer MINI Dialysis Unit?
0.3 µl/mm2, for a 10 µl sample.

What materials are used to manufacture the Slide-A-Lyzer MINI Dialysis Unit?
The membrane is regenerated cellulose and the plastic is a polypropylene copolymer. Both materials have very low protein and nucleic acid binding properties.

What percent of my sample should I expect to lose during dialysis?
In general, polypropylene and regenerated cellulose have very low nonspecific binding properties. The percent recovery of a sample will depend on the unique properties of the sample and on the initial concentration of the sample.

With what chemicals is the Slide-A-Lyzer MINI Dialysis Unit compatible?
Alcohols, ketones, esters, oxides and solvents containing nitrogen alkalis: may cause pore swelling or shrinkage w/ 1N sodium hydroxide or 1N ammonium hydroxide; not recommended for 1N potassium hydroxide Acids: will survive weak acids (100% acetic or formic acids), or ~25% phosphoric or sulfuric acids; the Slide-A-Lyzer MINI Dialysis Unit should not be used for >25% strong acids, although a weak solution of a strong acids may work.

 

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