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DyLight 800

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 800 is a near-IR fluor that is invisible to the naked eye but increases the staining options when using infrared imaging systems. DyLight 800 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor* 790 and IRDye* 800, and has high solubility for maximal incorporation of the dye into the biomolecule of interest.

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Fluorescent Probes

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DyLight Fluor Product Guide

DyLight 800 Properties and Applications

DyLight 800 spectral properties
Thermo Scientific DyLight 800 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (brown) spectra are normalized to the same height in this graph.

 

Properties of Thermo Scientific DyLight 800.
Parameter Value
Excitation / emission maxima 777nm / 794nm
Emission color Near Infrared
Molar extinction coefficient (ε) 270,000 M-1 cm-1
Correction factor (A280/A777) † 0.045
Molecular weight

NHS ester: 1050g/mol
Maleimide: 1075g/mol

Spectrally similar dyes Alexa Fluor* 790, IRDye* 800
† Correction factor can be used to estimate protein labeling efficiency.

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Emission spectra of DyLight Fluorescent Dyes.
Compare spectra and properties among all DyLight Fluors

 

Related pages:
DyLight 350
DyLight 405
DyLight 488
DyLight 550
DyLight 594
DyLight 633
DyLight 650
DyLight 680
DyLight 755
DyLight 800 (you are here)

 

In-cell ELISA DyLight 680 800 fluorophore fluorescence signal multiplex
Multiplex analysis of multiple targets using an In-Cell ELISA Near Infrared Detection Kit. Relative levels of total (red) and phosphorlylated (green) STAT3, STAT6 and EGFR were measured in untreated A431 cells and compared to cells treated with 100ng/mL EGF alone or EGF and 25 nM PD168393 (EGFR inhibitor). After probing with their respective primary antibodies, the total amount of each protein was fluorescently detected with a DyLight 680-Conjugated Secondary Antibody and counterstained for the phosphorylated form of each protein with DyLight 800-Conjugated Secondary Antibody. Cells were also labeled in the absence of primary antibody (no 1°) as a control for nonspecific binding of the secondary antibodies. Cells were imaged with the Odyssey* Infrared Imaging System using the 700 and 800 channels.

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Generic In-Cell ELISA Kit

Target-specific In-Cell ELISA Kits

  fluorescent fluorescence Western blot DyLight 680 800 siRNA Two-color infrared Western blot detection using Thermo Scientific DyLight 680- and DyLight 800-Conjugated Secondary Antibodies. p53 (53 kDa; red) and cyclophilin B (21 kDa; green) levels in response to siRNA expression in A549 cells were detected by Western blot analysis using DyLight 680- (red) and DyLight 800- (green) Conjugated Secondary Antibodies. Beta-catenin (90kDa, green) was used as the load control. Membrane was imaged with the Odyssey* Infrared Imaging System using the 700 and 800 channels.

 

 

DyLight 800 Products

DyLight 800 Reactive Dyes and Kits:

DyLight 800-conjugated Fluorescent Probes:

In-Cell ELISA Near Infrared Detection Kit

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DyLight Specialty Dyes

DyLight Fluor Product Guide

DyLight 800 References

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