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DyLight 755

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 755 replaces DyLight 750 as a near-IR fluor that is invisible to the naked eye but increases the staining options when using infrared imaging systems. DyLight 755 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor* 750, and has high solubility for maximal incorporation of the dye into the biomolecule of interest.

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Protein Methods Library Home


Fluorescent Probes

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DyLight Fluor Product Guide

DyLight 755 Properties and Applications

DyLight 755 excitation emission spectra spectrum
Thermo Scientific DyLight 755 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (brown) spectra are normalized to the same height in this graph.

 

Properties of Thermo Scientific DyLight 755.
Parameter Value
Excitation / emission maxima 754nm / 776nm
Emission color Near Infrared
Molar extinction coefficient (ε) 220,000 M-1 cm-1
Correction factor (A280/A754) † 0.030
Molecular weight

NHS ester: 1092g/mol
Maleimide: 1117g/mol

Spectrally similar dyes Alexa Fluor* 750
† Correction factor can be used to estimate protein labeling efficiency.

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Emission spectra of DyLight Fluorescent Dyes.
Compare spectra and properties among all DyLight Fluors

 

Related pages:
DyLight 350
DyLight 405
DyLight 488
DyLight 550
DyLight 594
DyLight 633
DyLight 650
DyLight 680
DyLight 755 (you are here)
DyLight 800

 

Fluorescent Western blot using DyLight 750 Infrared Western blot detection of ERK1 using Thermo Scientific DyLight 755-conjugated secondary antibody. Serial dilutions of A562 cell lysates were analyzed by Western blotting for levels of ERK1 using a mouse anti-ERK1 primary antibody and a goat anti-mouse secondary antibody conjugated to DyLight 755 (using Microscale Antibody Labeling Kit). Left lane is an infrared molecular weight marker.

 

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DyLight 755 Microscale Antibody Labeling Kit

DyLight 755 Products

DyLight 755 Reactive Dyes and Kits:

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DyLight Specialty Dyes

DyLight Fluor Product Guide

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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