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DyLight 680

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 680 produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5.5* and Alexa Fluor* 680, and is ideal for multi-color applications. DyLight 680 is available as reactive labeling agents and as conjugates of secondary antibodies and biotin-binding proteins for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.

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Fluorescent Probes

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DyLight Fluor Product Guide

DyLight 680 Properties and Applications

DyLight 680 spectra spectrum wavelength excitation emission
Thermo Scientific DyLight 680 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (red) spectra are normalized to the same height in this graph.

 

Properties of Thermo Scientific DyLight 680.
Parameter Value
Excitation / emission maxima 682nm / 715nm
Emission color Near Infrared
Molar extinction coefficient (ε) 140,000 M-1 cm-1
Correction factor (A280/A682) † 0.128
Molecular weight

NHS ester: 950g/mol
Maleimide: 972g/mol

Spectrally similar dyes Alexa Fluor* 680, Cy5.5*
† Correction factor can be used to estimate protein labeling efficiency.

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Emission spectra of DyLight Fluorescent Dyes.
Compare spectra and properties among all DyLight Fluors

 

Related pages:
DyLight 350
DyLight 405
DyLight 488
DyLight 550
DyLight 594
DyLight 633
DyLight 650
DyLight 680 (you are here)
DyLight 755
DyLight 800

 

fluorescence fluorescent DyLight 680 IR alexa fluor secondary antibody antibodies
Immunofluorescence microscopy using Thermo Scientific DyLight 680 dye. Cytokeratin 18 (pseudo-colored yellow) and lamin A (blue) in A549 cells were fluorescently labeled with their respective primary antibodies and DyLight 680-Conjugated Goat Anti-Rabbit or DyLight 405-Conjugated Goat Anti-Mouse Secondary Antibodies, respectively.
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Fluorescent Probes: Description, Methods and Applications
In-cell ELISA DyLight 680 800 fluorophore fluorescence signal multiplex
Multiplex analysis of multiple targets using an In-Cell ELISA Near Infrared Detection Kit. Relative levels of total (red) and phosphorlylated (green) STAT3, STAT6 and EGFR were measured in untreated A431 cells and compared to cells treated with 100ng/mL EGF alone or EGF and 25 nM PD168393 (EGFR inhibitor). After probing with their respective primary antibodies, the total amount of each protein was fluorescently detected with a DyLight 680-Conjugated Secondary Antibody and counterstained for the phosphorylated form of each protein with DyLight 800-Conjugated Secondary Antibody. Cells were also labeled in the absence of primary antibody (no 1°) as a control for nonspecific binding of the secondary antibodies. Cells were imaged with the Odyssey* Infrared Imaging System using the 700 and 800 channels.

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Generic In-Cell ELISA Kit

Target-specific In-Cell ELISA Kits

  fluorescent fluorescence Western blot DyLight 680 800 siRNA Two-color infrared Western blot detection using Thermo Scientific DyLight 680- and DyLight 800-Conjugated Secondary Antibodies. p53 (53 kDa; red) and cyclophilin B (21 kDa; green) levels in response to siRNA expression in A549 cells were detected by Western blot analysis using DyLight 680- (red) and DyLight 800- (green) Conjugated Secondary Antibodies. Beta-catenin (90kDa, green) was used as the load control. Membrane was imaged with the Odyssey* Infrared Imaging System using the 700 and 800 channels.

 

 

DyLight 680 Comparative Data

tubulin alpha DyLight 680 fluorescent fluorescence antibody antibodies secondary  cells tubulin fluorescence antibody fluorescent antibodies secondary DyLight 680 cells
Thermo Scientific DyLight 680 produces fluorescence intensity comparable to Alexa Fluor* 680. Cytokeratin 18 (red) in A549 cells was fluorescently labeled with a rabbit polyclonal anti-cytokeratin 18 antibody and DyLight 680 (left panel) or Alexa Fluor* 680 (right panel)-Conjugated Highly Cross-Adsorbed Goat Anti-Rabbit Secondary Antibody. Nuclei were counterstained with Hoechst dye. All images were captured using identical microscopic and camera settings.

 

DyLight 680 Products

DyLight 680 Reactive Dyes and Kits:

DyLight 680-conjugated Fluorescent Probes:

In-Cell ELISA Near Infrared Detection Kit

Related products:

DyLight Specialty Dyes

DyLight Fluor Product Guide

DyLight 680 References

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  2. Costford S. R. et al. (2010) Skeletal muscle nampt is induced by exercise in humans. Am J Physiol Endocrinol Metab. 298, E117-26.
  3. Groves B. et al. (2010) An inhibitory role of the g-protein regulator ags3 in mtor-dependent macroautophagy. PLoS One. 5, e8877.
  4. Groves B. et al. (2010) An inhibitory role of the g-protein regulator ags3 in mtor-dependent macroautophagy. PLoS One. 5, 421-9.
  5. Hassane S. et al. (2010) Elevated tgf–smad signalling in experimental pkd1 models and human patients with polycystic kidney disease. The Journal of Pathology. 222, 21-31.
  6. Himeda C. et al. (2010) Klf3 regulates muscle-specific gene expression and synergizes with serum response factor on klf binding sites. Molecular and cellular biology. 30, 3430.
  7. Hsiang T. Y. et al. (2009) Interferon-induced isg15 conjugation inhibits influenza a virus gene expression and replication in human cells. J Virol. 83, 5971-7.
  8. Liu Z. et al. (2010) Quasi-confocal, multichannel parallel scan hyperspectral fluorescence imaging method optimized for analysis of multicolor microarrays. Anal Chem. 82, 7752-7.
  9. Mistou M. Y. et al. (2009) Molecular dissection of the seca2 locus of group b streptococcus reveals that glycosylation of the srr1 lpxtg protein is required for full virulence. J Bacteriol. 191, 4195-206.
  10. Mizuno-Yamasaki E. et al. (2010) Phosphatidylinositol 4-phosphate controls both membrane recruitment and a regulatory switch of the rab gef sec2p. Developmental Cell. 18, 828-40.
  11. Nilsson C. et al. (2009) Quantitative phosphoproteomic analysis of the stat3/il-6/hif1 signaling network: An initial study in gsc11 glioblastoma stem cells. Journal of Proteome Research. 9, 430-43.
  12. Nookala S. et al. (2010) In search of the identity of the xap-1 antigen: A protein localized to cone outer segments. Invest Ophthalmol Vis Sci. 51, 2736-43.
  13. Paternot S. and Roger P. (2009) Combined inhibition of mek and mammalian target of rapamycin abolishes phosphorylation of cyclin-dependent kinase 4 in glioblastoma cell lines and prevents their proliferation. Cancer Research. 69, 4577.
  14. Petrucelli A. et al. (2009) Characterization of a novel golgi apparatus-localized latency determinant encoded by human cytomegalovirus. J Virol. 83, 5615.
  15. Rocha A. et al. (2008) Cyclic amp inhibits the proliferation of thyroid carcinoma cell lines through regulation of cdk4 phosphorylation. Molecular Biology of the Cell. 19, 4814.
  16. Rytinki M. and Palvimo J. (2008) Sumoylation modulates the transcription repressor function of rip140. J Biol Chem. 283, 11586.
  17. Senis Y. et al. (2009) The tyrosine phosphatase cd148 is an essential positive regulator of platelet activation and thrombosis. Blood. 113, 4942.
  18. Shih T. T. et al. (2009) Bone marrow angiogenesis magnetic resonance imaging in patients with acute myeloid leukemia: Peak enhancement ratio is an independent predictor for overall survival. Blood. 113, 3161-7.
  19. Sun Z. et al. (2010) The cysteine protease domain of porcine reproductive and respiratory syndrome virus nonstructural protein 2 possesses deubiquitinating and interferon antagonism functions. J Virol. 84, 7832.
  20. van Harn T. et al. (2010) Loss of rb proteins causes genomic instability in the absence of mitogenic signaling. Genes & development. 24, 1377.
  21. Wang X. et al. (2009) Proteomic analysis of the retina: Removal of rpe alters outer segment assembly and retinal protein expression. Glia. 57, 380-92.
  22. Wang X. et al. (2010) Cellular retinol binding protein 1 modulates photoreceptor outer segment folding in the isolated eye. Developmental Neurobiology. 70, 623-35.
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  24. Yim V. et al. (2008) Multipurpose dvd pick-up scanner for analysis of microfluidics and micromechanical structures. Conf Proc IEEE Eng Med Biol Soc. 2008, 2749-51.

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