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DyLight 633

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 633 fluoresces red with physical properties comparable to other 633 dyes. With stability over a wide pH range and higher solutility than traditional fluorescent dyes, DyLight 633 is ideal for multi-color applications. DyLight 633 is available as reactive labeling agents and as conjugates of secondary antibodies and biotin-binding proteins for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.

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Fluorescent Probes

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DyLight Fluor Product Guide

DyLight 633 Properties and Applications

DyLight 633 wavelength excitation emission infrared spectrum spectra far red
Thermo Scientific DyLight 633 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (red) spectra are normalized to the same height in this graph.


Properties of Thermo Scientific DyLight 633.
Parameter Value
Excitation / emission maxima 638nm / 658nm
Emission color Red
Molar extinction coefficient (ε) 170,000 M-1 cm-1
Correction factor (A280/A638) † 0.110
Molecular weight

NHS ester: 1066g/mol
Maleimide: 1091g/mol

Spectrally similar dyes Alexa Fluor* 633
† Correction factor can be used to estimate protein labeling efficiency.

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Emission spectra of DyLight Fluorescent Dyes.
Compare spectra and properties among all DyLight Fluors


Related pages:
DyLight 350
DyLight 405
DyLight 488
DyLight 550
DyLight 594
DyLight 633 (you are here)
DyLight 650
DyLight 680
DyLight 755
DyLight 800


antibody cytokeratin antibodies secondary DyLight 633  fluorescent fluoresence red tissue carconima colon
antibody cytokeratin lamin nucleus nuclear antibodies DyLight 633 405 fluorescence secondary  far red
Immunofluorescence microscopy using Thermo Scientific DyLight 633 dye. Top panel: Cytokeratin 18 (red) in human colon carcinoma tissue was fluorescently detected with a biotinylated anti-cytokeratin 18 antibody and Pierce Streptavidin-Conjugated DyLight 633. Thermo Scientific Pierce Hoechst stain was also used to fluorescently label cell nuclei.. Bottom panel: Cytokeratin 18 (red) and lamin A (blue) in A549 cells were fluorescently labeled using specific primary antibodies and DyLight 633-Conjugated Highly-Cross Adsorbed Anti-Rabbit and DyLight 405-Conjugated Anti-Mouse Secondary Antibodies, respectively.


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Fluorescent Probes: Description, Methods and Applications

DyLight 633 Products

DyLight 633 Reactive Dyes and Kits:

DyLight 633-conjugated Fluorescent Probes:

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DyLight Specialty Dyes

DyLight Fluor Product Guide

DyLight 633 References

  1. Feng D. et al. (2010) Cellular internalization of exosomes occurs through phagocytosis. Traffic. 11, 675-87.
  2. Fietz S. A. et al. (2010) Osvz progenitors of human and ferret neocortex are epithelial-like and expand by integrin signaling. Nat Neurosci. 13, 690-9.
  3. Kronenberg D. et al. (2010) Processing of procollagen iii by meprins: New players in extracellular matrix assembly? J Invest Dermatol. 130, 2727-35.
  4. Li D. et al. (2010) Molecular imaging of atherosclerotic plaques targeted to oxidized ldl receptor lox-1 by spect/ct and magnetic resonance. Circ Cardiovasc Imaging. 3, 464-72.
  5. McCormick P. et al. (2009) Impaired recruitment of grk6 and -arrestin2 causes delayed internalization and desensitization of a whim syndrome-associated cxcr4 mutant receptor. PLoS One. 4, 9448-53.
  6. Nichols M. D. et al. (2009) Factors affecting size and swelling of poly(ethylene glycol) microspheres formed in aqueous sodium sulfate solutions without surfactants. Biomaterials. 30, 5283-91.
  7. Roam J. et al. (2010) The formation of protein concentration gradients mediated by density differences of poly (ethylene glycol) microspheres. Biomaterials.
  8. Scott E. A. et al. (2010) Modular scaffolds assembled around living cells using poly(ethylene glycol) microspheres with macroporation via a non-cytotoxic porogen. Acta Biomater. 6, 29-38.
  9. Thygesen L. G. et al. (2010) Role of supramolecular cellulose structures in enzymatic hydrolysis of plant cell walls. J Ind Microbiol Biotechnol.

Related literature...
Thermo Scientific Pierce Fluorescent Products Guide
Fluorescent Labeling and Detection Products Guide


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