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DyLight 594

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 594 generates red fluorescence and shows wide excitation wavelength separation from green fluorophores for multi-color applications with reduced bleedthrough. The physical characteristics of DyLight 594 are comparable to other 594 dyes, is stable over a wide pH range and exhibits higher solutility than traditional fluorescent dyes. DyLight 594 is available as reactive labeling agents and as conjugates of secondary antibodies and biotin-binding proteins for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.

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Fluorescent Probes

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DyLight Fluor Product Guide

DyLight 594 Properties and Applications

DyLight 594 wavelength excitation emission infrared spectrum spectra red
Thermo Scientific DyLight 594 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (orange) spectra are normalized to the same height in this graph.


Properties of Thermo Scientific DyLight 594.
Parameter Value
Excitation / emission maxima 593nm / 618nm
Emission color Red
Molar extinction coefficient (ε) 80,000 M-1 cm-1
Correction factor (A280/A593) † 0.585
Molecular weight

NHS ester: 1078g/mol
Maleimide: 1059g/mol

Spectrally similar dyes Alexa Fluor* 594, Texas Red*
† Correction factor can be used to estimate protein labeling efficiency.

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Emission spectra of DyLight Fluorescent Dyes.
Compare spectra and properties among all DyLight Fluors


Related pages:
DyLight 350
DyLight 405
DyLight 488
DyLight 550
DyLight 594 (you are here)
DyLight 633
DyLight 650
DyLight 680
DyLight 755
DyLight 800


antibody cytokeratin antibodies secondary DyLight 594  fluorescent fluoresence red tissue carconima colon
antibody cytokeratin  antibodies DyLight 594fluorescence secondary red
Immunofluorescence microscopy using Thermo Scientific DyLight 594 dye. Top panel: Cytokeratin 18 (red) in human colon carcinoma tissue was fluorescently detected with a biotinylated anti-cytokeratin 18 antibody and Thermo Scientific Pierce Streptavidin-Conjugated DyLight 594. Bottom panel: Cytokeratin 18 (red) in A549 cells was fluorescently labeled using an anti-cytokeratin 18 primary antibody and DyLight 594-Conjugated Anti-Rabbit Secondary Antibody. In both panels, Thermo Scientific Pierce Hoechst stain was also used to fluorescently label cell nuclei.


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Fluorescent Probes: Description, Methods and Applications

DyLight 594 Products

DyLight 594 Reactive Dyes and Kits:

DyLight 594-conjugated Fluorescent Probes:

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DyLight Specialty Dyes

DyLight Fluor Product Guide

DyLight 594 References

  1. Chen Y. et al. (2010) Hepatic differentiation of liver derived progenitor cells and their characterization by microrna analysis. Liver Transplantation. 16, 1086-97.
  2. Ciruela F. et al. (2010) Evidence for oligomerization between gabab receptors and girk channels containing the girk1 and girk3 subunits. Eur J Neurosci. 32, 1265-77.
  3. Dimitrov E. and Usdin T. B. (2010) Tuberoinfundibular peptide of 39 residues modulates the mouse hypothalamic-pituitary-adrenal axis via paraventricular glutamatergic neurons. J Comp Neurol. 518, 4375-94.
  4. Ferrati S. et al. (2010) Intracellular trafficking of silicon particles and logic-embedded vectors. Nanoscale. 2, 1512-20.
  5. Haces M. et al. (2010) Selective vulnerability of brain regions to oxidative stress in a non-coma model of insulin-induced hypoglycemia. Neuroscience. 165, 28-38.
  6. Huang J. et al. (2009) Retinoic acid signalling induces the differentiation of mouse fetal liver derived hepatic progenitor cells. Liver International. 29, 1569-81.
  7. Lei X. et al. (2010) The 3c protein of enterovirus 71 inhibits retinoid acid-inducible gene i-mediated interferon regulatory factor 3 activation and type i interferon responses. Journal of Virology. 84, 8051.
  8. Ouellet E. et al. (2010) Parallel microfluidic surface plasmon resonance imaging arrays. Lab on a Chip. 10, 581-8.
  9. Rose E. et al. (2009) Glutamate transporter coupling to na, k-atpase. Journal of Neuroscience. 29, 8143.
  10. Sarkar P. et al. (2010) Photophysical properties of a new dylight 594 dye. Journal of Photochemistry and Photobiology B: Biology. 98, 35-9.
  11. Serda R. et al. (2009) Quantitative mechanics of endothelial phagocytosis of silicon microparticles. Cytometry Part A. 75, 752-60.
  12. Singh S. K. et al. (2010) Tumour-associated glycan modifications of antigen enhance mgl2 dependent uptake and mhc class i restricted cd8 t cell responses. Int J Cancer.
  13. Veenstra J. (2009) Peptidergic paracrine and endocrine cells in the midgut of the fruit fly maggot. Cell and Tissue Research. 336, 309-23.
  14. Wei L. et al. (2009) Domain-and species-specific monoclonal antibodies recognize the von willebrand factor-c domain of ccn5. Journal of Cell Communication and Signaling. 3, 65-77.
  15. Wei L. et al. (2010) A monoclonal antibody approach to ccn5 domain analysis. CCN proteins in health and disease, 77-95.
  16. Zhu G. et al. (2009) Activation of rxr and rar signaling promotes myogenic differentiation of myoblastic c2c12 cells. Differentiation. 78, 195–204.

Related literature...
Thermo Scientific Pierce Fluorescent Products Guide
Fluorescent Labeling and Detection Products Guide

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