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DyLight 550

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 550 replaces DyLight 549 to provide vibrant orange-to-red fluorescence with better performance than other rhodamine derivatives, including Alexa Fluor* 555, TRITC and Cy3* for fluorescent applications. DyLight 550 has tight excitation/emission spectra that are ideal for multicolor applications. DyLight 550 is available as reactive labeling agents for custom labeling applications. DyLight 550-conjugated secondary antibodies and biotin-binding proteins are also available for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.

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Fluorescent Probes

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DyLight Fluor Product Guide

DyLight 550 Properties and Applications

DyLight 550 spectral characteristics
Thermo Scientific DyLight 550 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (orange) spectra are normalized to the same height in this graph.

 

Properties of Thermo Scientific DyLight 550.
Parameter Value
Excitation / emission maxima 562nm / 576nm
Emission color Orange to Red
Molar extinction coefficient (ε) 150,000 M-1 cm-1
Correction factor (A280/A562) † 0.081
Molecular weight

NHS ester: 1040g/mol
Maleimide: 1065g/mol

Spectrally similar dyes Alexa Fluor* 555, Cy3, TRITC
† Correction factor can be used to estimate protein labeling efficiency.

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Emission spectra of DyLight Fluorescent Dyes.
Compare spectra and properties among all DyLight Fluors

 

Related pages:
DyLight 350
DyLight 405
DyLight 488
DyLight 550 (you are here)
DyLight 594
DyLight 633
DyLight 650
DyLight 680
DyLight 755
DyLight 800

 

Fluorescent detection of protein disulfide isomerase (PDI) in U2OS cells with DyLight 550 dye
Fluorescent detection of cytokeratin 18 and lamin B1 in U2OS cells with DyLight 550 and 650 dyes, respectively.
Immunofluorescence microscopy using Thermo Scientific DyLight 550 dye. Top panel: Protein disulfide isomerase (PDI; red) in U2OS cells was detected with an anti-PDI primary antibody and goat anti-mouse secondary antibody labeled with the DyLight 550 Microscale Antibody Labeling Kit. Nuclei were labeled with Hoechst dye. Bottom panel: Cytokeratin 18 (green) and lamin B1(red) in U2OS cells were detected with the respective primary antibodies and goat anti-mouse and anti-rabbit secondary antibodies labeled with DyLight 550 and 650 Microscale Antibody Labeling Kits, respectively.

 

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Fluorescent Probes: Description, Methods and Applications

 

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Thermo Scientific Pierce Fluorescent Products Guide
Fluorescent Labeling and Detection Products Guide

DyLight 550 Comparative Data

Improved fluorescent detection of lamin A and lamin B1 with DyLight 550 compared to Alexa Fluor 555 and Cy3 dyes
Thermo Scientific DyLight 550 dye produces intense fluorescent staining that is comparable to our retired DyLight 549 dye and brighter than other commercially available dyes. The nuclear envelope in A549 cells was fluorescently labeled with anti-lamin A (top row) or anti-lamin B1 (bottom row) primary antibodies and commercially available goat anti-mouse or anti-rabbit IgG secondary antibodies, respectively, that were conjugated to Thermo Scientific DyLight 550, the retired DyLight 549, Alexa Fluor 555 or Cy3 dye. All images were captured using identical microscopic and camera settings and were processed identically.

 

Improved fluorescent detection of NF-kB with DyLight 550 compared to Alexa Fluor 555 and Cy3 dyes
Thermo Scientific DyLight 550 dye produces intense NF-kB staining that is comparable to our retired DyLight 549 dye and brighter than other commercially available dyes. NF-kB (orange) in A549 cells was fluorescently labeled with an anti-NFkB primary antibody and a commercially available goat anti-mouse IgG secondary antibody conjugated to DyLight 550, the retired DyLight 549, Alexa Fluor 555 or Cy3 dye. Nuclei (blue) were stained with Hoechst dye. All images were captured using identical microscopic and camera settings and were processed identically.

 

DyLight 550 Products

DyLight 550 Reactive Dyes and Kits:

DyLight 550-conjugated Fluorescent Probes:

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DyLight Specialty Dyes

DyLight Fluor Product Guide
Fluorescent detection of lamin A with DyLight 550 dye DyLight 550 is comparable to DyLight 549 in the fluorescent detection of lamin A DyLight 550 shows stronger fluorescent detection of lamin A than Alexa Fluor 555 dye DyLight 550 shows stronger fluorescent detection of lamin A than Cy3 dye Fluorescent detection of lamin B1 with DyLight 550 dye DyLight 550 is comparable to DyLight 549 in the fluorescent detection of lamin B1 DyLight 550 shows stronger fluorescent detection of lamin B1 than Alexa Fluor 555 dye DyLight 550 shows stronger fluorescent detection of lamin B1 than Cy3 dye Fluorescent detection of NF-kB with DyLight 550 dye DyLight 550 is comparable to DyLight 549 in the fluorescent detection of NF-kB DyLight 550 shows stronger fluorescent detection of NF-kB than Alexa Fluor 555 dye DyLight 550 shows stronger fluorescent detection of NF-kB than Cy3 dye
Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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