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DyLight 488

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 488 provides exceptional performance for a wide array of fluorescence-base applications at a lower cost than other 488-range dyes. DyLight 488 shows greater photostability than traditional fluorescein-based dyes and has a greater range of pH insensitivity (pH 4-9). DyLight 488 is available as reactive labeling agents and as conjugates of secondary antibodies and biotin-binding proteins for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.

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Fluorescent Probes

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DyLight Fluor Product Guide

DyLight 488 Properties and Applications

DyLight 488 Alexa fluor spectra spectrum excitation emission absorbance wavelength
Thermo Scientific DyLight 488 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (green) spectra are normalized to the same height in this graph.

 

Properties of Thermo Scientific DyLight 488.
Parameter Value
Excitation / emission maxima 493nm / 518nm
Emission color Green
Molar extinction coefficient (ε) 70,000 M-1 cm-1
Correction factor (A280/A493) † 0.147
Molecular weight

NHS ester: 1011g/mol
Maleimide: 800g/mol

Spectrally similar dyes Alexa Fluor* 488, Cy2*, FITC
† Correction factor can be used to estimate protein labeling efficiency.

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Emission spectra of DyLight Fluorescent Dyes.
Compare spectra and properties among all DyLight Fluors

 

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DyLight 488 (you are here)
DyLight 550
DyLight 594
DyLight 633
DyLight 650
DyLight 680
DyLight 755
DyLight 800
fluorescence antibody antibodies lamin DyLight 488 fluorescent microscopy A549 cells secondary Hoechst dye
fluorescent fluorescence phospho-EGFR phosphorylated DyLight 488 secondary antibody antibodies Hoechst dye
Immunofluorescence microscopy using Thermo Scientific DyLight 488 dye. Top panel: Cytokeratin 18 (red) and Lamin A (green) in A549 cells were fluorescently labeled with specific primary antibodies and DyLight 594-Conjugated Highly Cross-Adsorbed Goat Anti-Rabbit or DyLight 488-Conjugated Goat Anti-Mouse Secondary Antibodies, respectively. Bottom panel: Phosphorylated EGFR (green) and nuclei (blue) of A431 cells were labeled with a specific primary antibody and DyLight 488-Conjugated Goat Anti-Mouse Secondary Antibody or Hoechst dye, respectively .

 

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Fluorescent Probes: Description, Methods and Applications

DyLight 488 Comparative Data

cytokeratin DyLight 488 Cy2 fluorescent fluorescence antibody antibodies secondary  cells cytokeratin fluorescence antibody fluorescent antibodies secondary DyLight 488 FITC cells
DyLight 488 Alexa fluor fluorescent fluorescence antibody antibodies secondary DyLight 488 cytokeratin antibody antibodies secondary fluorescence fluorescent
Thermo Scientific DyLight 488 produces intense fluorescent staining substantially brighter than many commercially available dyes. Intermediate filaments in A549 cells (green) were fluorescently labeled with a rabbit polyclonal anti-cytokeratin 18 antibody and goat anti-rabbit IgG secondary antibodies conjugated to Cy2*, fluorescein (FITC), Alexa Fluor* 488 or DyLight 488 (Part No. 35552). Nuclei were counterstained with Hoechst dye. All images were captured using identical microscopic and camera settings.

 

DyLight 488 Products

DyLight 488 Reactive Dyes and Kits:

DyLight 488-conjugated Fluorescent Probes:

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DyLight Specialty Dyes

DyLight Fluor Product Guide

DyLight 488 References

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