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DyLight 405

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 405 provides exceptional performance for a wide array of fluorescence-base applications at a lower cost than other 405-range dyes. DyLight 405 has a narrow excitation wavelength that makes the dye ideal for multi-color applications and is available as a reactive labeling agent and a conjugate of secondary antibodies and biotin-binding proteins for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.

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Fluorescent Probes

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DyLight Fluor Product Guide

DyLight 405 Properties and Applications

DyLight 488 spectra spectrum spectral wavelength excitation emission color
Thermo Scientific DyLight 405 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (blue) spectra are normalized to the same height in this graph.


Properties of Thermo Scientific DyLight 405.
Parameter Value
Excitation / emission maxima 400nm / 420nm
Emission color Blue
Molar extinction coefficient (ε) 30,000 M-1 cm-1
Correction factor (A280/A400) † 0.564
Molecular weight

NHS ester: 793g/mol
Maleimide: 818g/mol

Spectrally similar dyes Alexa Fluor* 405, Cascade* blue, AMCA
† Correction factor can be used to estimate protein labeling efficiency.

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Emission spectra of DyLight Fluorescent Dyes.
Compare spectra and properties among all DyLight Fluors


Related pages:
DyLight 350
DyLight 405 (you are here)
DyLight 488
DyLight 550
DyLight 594
DyLight 633
DyLight 650
DyLight 680
DyLight 755
DyLight 800
fluorescent fluorescence antibody antibodies secondary A549 DyLight 405 499 lamin cytokeratin
antibody antibodies DyLight 405 549 secondary vimentin lamin tissue IHC
Immunofluorescence microscopy using Thermo Scientific DyLight 405 dye. Top panel: Cytokeratin 18 (blue) and lamin A (green) in A549 cells were fluorescently labeled with specific primary antibodies and DyLight 405-Conjugated Highly Cross-Adsorbed Goat Anti-Rabbit or DyLight 488-Conjugated Goat Anti-Mouse Secondary Antibodies, respectively. Bottom panel: Vimentin (red) and lamin B1 (blue) in normal human colon tissue were fluorescently labeled with specific primary antibodies and DyLight 405-Conjugated Goat Anti-Mouse or DyLight 549-Conjugated Goat Anti-Rabbit Secondary Antibodies, respectively. Cell nuclei were counterstained green.


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Fluorescent Probes: Description, Methods and Applications

DyLight 405 Products

DyLight 405 Reactive Dyes and Kits:

DyLight 405-conjugated Fluorescent Probes:

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DyLight Specialty Dyes

DyLight Fluor Product Guide

DyLight 405 References

  1. Currier, T. A. et al. (2010) The Effects of Embryonic Knockdown of the Candidate Dyslexia Susceptibility Gene Homologue Dyx1c1 on the Distribution of Gabaergic Neurons in the Cerebral Cortex. Neuroscience
  2. Dimitrov, E. and Usdin, T. B. (2010) Tuberoinfundibular peptide of 39 residues modulates the mouse hypothalamic pituitary adrenal axis via paraventricular glutamatergic neurons. The Journal of Comparative Neurology.
  3. McNerny, D. Q. et al. (2009) RGD Dendron Bodies; Synthetic Avidity Agents with Defined and Potentially Interchangeable Effector Sites That Can Substitute for Antibodies. Bioconjugate chemistry 20, 1853-9.
  4. Rotzius, P. et al. (2010) Distinct Infiltration of Neutrophils in Lesion Shoulders in ApoE-/- Mice. Am. J. Pathol. 177, 493-500.
  5. Speidl, W. S. et al. (2010) The complement component C5a is present in human coronary lesions in vivo and induces the expression of MMP-1 and MMP-9 in human macrophages in vitro. FASEB J fj.
  6. Zhang, X. and Wong, S. M. (2009) Hibiscus chlorotic ringspot virus upregulates plant sulfite oxidase transcripts and increases sulfate levels in kenaf (Hibiscus cannabinus L.). J. Gen. Virol. 90, 3042-50.

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Thermo Scientific Pierce Fluorescent Products Guide
Fluorescent Labeling and Detection Products Guide

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